Development of next generation nano-biomaterials by cell surface engineering

点击翻译按钮获取中文摘要

We tried an approach to construct 2- and 3-dimensional tissues using a ligand-immobilized polymer chain as an artificial extracellular matrix. This technique based on the cell-agglomerate engineering allowed the efficient cellular coating of artificial materials with complicated shapes for improvement of biocompatibility and facilitation of tissue reconstruction. Next, we proposed a novel method for enhancing ligand-receptor binding and modifying cell surface as artificial material. So, we prepared a multi-functional polymer (polymer nano-tool) consisted of ligand, photo-reactive group, biotin and disulfide. We found that cells modified with biotin-carrying nano-tools specifically adhered to an avidin-immobilized plate and the formation of cell agglomerates was mediated by adding avidin.