The establishment of an analytical method to evaluate of the rehabilitation with biogenic specimens obtained from rehabilitation patient and disease model animal

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Using the serum of the inpatient and the muscle of exercise-load animal, We examined analyses of protein and amino acid in the specimens with a biochemical technique to evaluate the effect of the rehabilitation training.The magnified expression of a protein spot of the serum that fluctuated on and admission and discharge from hospital was observed by two-dimension electrophoresis.In addition an increase and decrease in the analysis of free amino acid were observed using UPLC, although any specific component that linked with the rehabilitation exercise was not identified . On the one hand there was a correlation between HGF and FIM efficiencies in the determination of cytokines.