Development of microbial catalyst catalyzing high shikimate production from quinate

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A novel strategy preparing high shikimic acid with high efficiency with developed microbial enzymes from fungi and acetic acid bacteria has finally completed during three years of investigation supported by this grant. Coffee pulp koji facilitated strong formation of chlorogenic acid hydrolase and yielded quinic acid from coffee pulp easier than any other methods so far reported. Quinic acid was converted by two enzyme systems of acetic acid bacteria, oxidative fermentation of 3-dehydroshikimate from quinic acid and asymmetric reduction yielding shikimic acid from 3-dehydroshikimate were so successful. The fruitful outcomes in this project must help people from pandemic flu through plentiful supply of Tamiflu synthesized from shikimic acid provided in this project.