Development of microbial catalyst catalyzing high shikimate production from quinate

催化奎宁酸高产莽草酸的微生物催化剂的研制

• 批准号: 19380050
• 负责人: ADACHI Osao
• 金额: $ 13.06万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380050/
• 关键词: 酢酸菌; 酸化発酵; シキミ酸; コーヒー麹; タミフル; キナ酸の製造; シキミ酸の製造; コーヒー粕麹; 固定化触媒

A novel strategy preparing high shikimic acid with high efficiency with developed microbial enzymes from fungi and acetic acid bacteria has finally completed during three years of investigation supported by this grant. Coffee pulp koji facilitated strong formation of chlorogenic acid hydrolase and yielded quinic acid from coffee pulp easier than any other methods so far reported. Quinic acid was converted by two enzyme systems of acetic acid bacteria, oxidative fermentation of 3-dehydroshikimate from quinic acid and asymmetric reduction yielding shikimic acid from 3-dehydroshikimate were so successful. The fruitful outcomes in this project must help people from pandemic flu through plentiful supply of Tamiflu synthesized from shikimic acid provided in this project.

在该基金支持下，经过三年的研究，最终完成了一种利用从真菌和醋酸菌中开发的微生物酶高效制备高莽草酸的新策略。咖啡浆曲促进了绿原酸水解酶的强烈形成，并且比迄今报道的任何其他方法更容易从咖啡浆中产生奎尼酸。利用醋酸菌的两种酶系转化奎宁酸，成功地将奎宁酸氧化发酵为3-脱氢莽草酸，并将3-脱氢莽草酸不对称还原为莽草酸。该项目的丰硕成果将通过提供大量由莽草酸合成的达菲来帮助人们免受流感大流行的影响。

项目成果

Coffee pulp koji of Aspergillus sojae as stable immobilized catalyst of chlorogenate hydrolase

酱油曲霉咖啡浆曲作为绿原酸水解酶的稳定固定化催化剂

• 发表时间: 2008
• 期刊: Appl. Microbiol. Biotechnol. 81
• 作者: O. Adachi;ら
• 通讯作者: ら

酢酸菌の固定化酵素を用いたシキミ酸製造法の検討

醋酸菌固定化酶生产莽草酸方法的研究

• 发表时间: 2011
• 作者: 足立収生;阿野嘉孝;品川恵美子;薬師寿治;松下一信
• 通讯作者: 松下一信

A novel 3-dehydroquinate dehydratase catalyzing extracellular formation of 3-dehydroshikimate by oxidative fermentation of Gluconobacter oxydans IFO 3244.

一种新型 3-脱氢奎宁酸脱水酶，通过氧化葡萄糖杆菌 IFO 3244 的氧化发酵，催化细胞外形成 3-脱氢莽草酸。

• 发表时间: 2008
• 期刊: Biosci. Biotechnol. Biochem. 72
• 作者: O. Adachi;Y. Ano;H. Toyama;K. Matsushita
• 通讯作者: K. Matsushita

Quinic acid prodouction from coffee pulp by fungal enzyme and subsequent conversion of quinic acid to shikimim acid by two enzymatic systems of acetic acid bacteria

通过真菌酶从咖啡果肉中生产奎宁酸，然后通过乙酸细菌的两个酶促系统将奎宁酸转化为莽草酸

• 发表时间: 2009
• 作者: 野田悟子;Eric Viscogliosi;大熊盛也;O.Adachi
• 通讯作者: O.Adachi

Formaldehyde elimination with formaldehyde and formate oxidase in membrane of acetic acid bacteria

• DOI: 10.1263/jbb.105.292
• 发表时间: 2008-03-01
• 期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
• 影响因子: 2.8
• 作者: Shinagawa, Emiko;Toyama, Hirohide;Adachi, Osao
• 通讯作者: Adachi, Osao