权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Quinoprotein-dependent periplasmic oxidase system in aerobic bacteria

需氧细菌中奎宁蛋白依赖性周质氧化酶系统

基本信息

批准号：
07044324
负责人：
ADACHI Osao
金额：
--
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for International Scientific Research.
财政年份：
1995
资助国家：
日本
起止时间：
1995 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044324/
关键词：
Pyrroloquinoline quinone Quinoprotein Electron transport chain Periplasmic oxidase

项目摘要

One year collaboration with three different institutes from Germany and the Netherlands has yielded the following results.(1) A novel bacterial strain, Pseudomonas putida HK5, was isolated from soil by enrichment technique. Three distinct quinoprotein alcohol dehydrogenases are expressed when grown on different alcohols. They are one quinoprotein and two quinohemoproteins and they showed interesting aspects in biochemistry, physiology and applied enzymology, on the basis of periplasmic oxidase.(2) Glucose dehydrogenase, a typical quinoprotein, is divided into two species depending on their localization, membrane-bound and soluble enzymes. The two enzymes were highly purified and converted to the apo-enzymes. The binding process of PQQ to the apo-enzymes was investigated revealing that the two enzymes are completely different each other in many aspects.(3) An inactive enzyme of alcohol dehydrogenase has been purified from the membrane fraction of Gluconobacter suboxydans. It is interesting very much to know that the inactive enzyme can be converted to the active one by the expenses of biological energy.(4) Alcohol dehydrogenase from Gluconobacter suboxydans is consisted of three different subunits. The second subunit contains three moles of cytochromes c and shows an interesting phenomena during the catalytic activity of alcohol oxidation. Several evidence for intramolecular flow of electron during enzyme activity were presented for the first time.

与来自德国和荷兰的三个不同研究所进行了为期一年的合作，取得了以下成果。(1)采用富集技术从土壤中分离到一株新的细菌-恶臭假单胞菌HK 5。当在不同的醇上生长时，表达三种不同的醌蛋白醇脱氢酶。它们是一种醌蛋白和两种醌血红素蛋白，在周质氧化酶的基础上，在生物化学、生理学和应用酶学方面显示出有趣的方面。(2)葡萄糖脱氢酶是一种典型的醌蛋白，根据其定位可分为膜结合酶和可溶性酶两种。这两种酶被高度纯化并转化为脱辅基酶。研究了PQQ与脱辅基酶的结合过程，发现这两种酶在许多方面是完全不同的。(3)一种失活的乙醇脱氢酶已从低氧化葡糖醛酸菌的膜组分中纯化出来。很有意思的是，不活跃的酶可以通过消耗生物能量转化为活跃的酶。(4)亚氧化葡糖酵母醇脱氢酶由三个不同的亚基组成。第二个亚基含有3摩尔的细胞色素c，并在醇氧化的催化活性过程中显示出有趣的现象。首次提出了酶活性过程中分子内电子流动的若干证据。