Molecular classification of hybrid larch seedling and origin of larch in Hokkaido Island

落叶松杂交苗的分子分类及北海道落叶松的起源

• 批准号: 19380093
• 负责人: TSUMURA Yoshihiko
• 金额: $ 12.23万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380093/
• 关键词: カラマツ雑種; DNA識別; オルガネラDNA; 北海道; 精英樹; 起源推定; 起原推定

Various hybrids between Larix species have been used in commercial forestry around the world because they have heterotic advantages. In Japan, a hybrid between Kurile larch (Larix gmelinii var.japonica) and Japanese larch (L.kaempferi) is one of the most important afforestation species in Hokkaido Island. This hybrid larch combines desirable traits of both parental species, including fast juvenile growth, stem straightness and relatively strong resistance to gnawing by Myodes rufocanus bedfordiae. Therefore, it is important to distinguish the hybrid seedlings from pure L.gmelinii var.japonica × L.gmelinii (GG) seedlings. We identified diagnostic chloroplast DNA and mitochondrial DNA markers that can (i) discriminate between L.gmelinii var.japonica and L.kaempferi, and (ii) determine the maternal and paternal species of hybrids between them, by exploiting the difference in inheritance mode between the two genomes. We also investigated the hybridization rates at a site with two types of … More inter-specific seed orchard.-a new type with rows of a single maternal clone of L.gmelinii var japonica amongst rows of L.kaemferi, and a "traditional" type with multiple, intimately mixed clones-in two years using chloroplast diagnostic DNA markers. The average hybridization rates in the SMC inter-specific seed orchards were higher than that in the traditional inter-specific seed orchards, because of the self-incompatibility of the L.gmelinii var. japonica clone. We detected significant differences in hybridization rates between the orchard types in both investigated years (P<0.001, ANOVA). This finding suggests that SMC inter-specific seed orchards can reliably provide seeds with high proportions of hybrids. The discriminant analysis revealed that root collar diameter and number of sylleptic branches at 2-years-old, and day of terminal bud set at land 2-yaers-old have high ability for hybrid classification of individual seedlings. These traits will be worked for improving the common method. The genetic structure of natural population of this species was weak but Mt. Manokami population has unique genetic diversity by both of SSRs and mitocondorial DNA analyses. The origin of plus tree population in Hokkaido Island was thought to be central Japan such as Kawakami village of Nagano prefecture by DNA analysis. Less

落叶松属树种间的各种杂交种由于具有杂种优势而被广泛应用于世界各地的商品林业。在日本，由日本落叶松和日本落叶松杂交而成的千岛落叶松是北海道岛最重要的造林树种之一。这种杂交落叶松结合了两个亲本的优良性状，包括快速的幼龄生长，茎直和相对较强的抵抗力鼠啮。因此，区分光叶百合杂种苗与光叶百合（GG）杂种苗具有重要意义。我们确定了诊断叶绿体DNA和线粒体DNA标记，可以（i）区分L.gmelinii var.japonica和L.kaempferi，和（ii）确定它们之间的杂种的母本和父本物种，通过利用两个基因组之间的遗传模式的差异。我们还调查了在一个地点与两种类型的杂交率 ...更多信息 种间种子果园一个新的类型与行中的一个单一的母系克隆的L.gmelinii变种日本L.kaemferi行，和一个“传统”的类型与多个，密切混合的克隆-在两年内使用叶绿体诊断DNA标记。SMC种间种子园的平均杂交率高于传统种间种子园，这是由于落叶松的自交不亲和性造成的。日本无性系两个调查年度果园类型之间的杂交率差异极显著（P<0.001，方差分析）。这一发现表明，SMC种间种子园可以可靠地提供种子与高比例的杂交种。判别分析表明，2年生根颈直径、二次枝数和地上2年生顶芽着生天数对单株杂种分类能力较强。这些特点将为改进常规方法提供参考。该种自然居群遗传结构较弱，但Mt.通过SSR和线粒体DNA分析，马诺卡米群体具有独特的遗传多样性。通过DNA分析，认为北海道岛的优树种群起源于日本中部地区，如长野县川上村。少

项目成果

Enhanced hybridization rates in a Larix gmelinii var. japonica x L. kaempferi inter-specific seed orchard with a single maternal clone revealed by cytoplasmic DNA markers.

提高落叶松 (Larix gmelinii) 变种的杂交率。

• 发表时间: 2008
• 期刊: Tree Genetics and Genomics 4
• 作者: Moriguchi;Y.;K. Kita;K. Uchiyama;M. Kuromaru;Y. Tsumura
• 通讯作者: Y. Tsumura

グイマツ雑種F1苗木の雑種率の年次変動と選苗精度

日本松杂交F1代苗杂交率和选苗准确率的年际变化

• 发表时间: 2009
• 作者: 来田和人・内山和子・市村康裕;黒丸亮・森口喜成・津村義彦
• 通讯作者: 黒丸亮・森口喜成・津村義彦

Evaluation of genetic diversity and provenance identification of Japanese larch plus trees in Hokkaido basic breeding region

北海道基本繁育区落叶松遗传多样性评价及种源鉴定

• 发表时间: 2010
• 作者: San Jose-Maldia;L.;Kita;K.;Tsumura;Y.;Kuromaru;M.;Tomaru;N
• 通讯作者: N

Verification of the morphological distinction method of hybrid larch seeding by DNA marker

DNA标记杂交落叶松播种形态区分方法验证

• 发表时间: 2007
• 作者: K. Kita;K. Uchiyama;Y. Moriguchi;M. Takagaki;Y. Saito;Y. Ide;Y. Tsumura;M. Kuromaru
• 通讯作者: M. Kuromaru

Mitochondrial DNA variation in natural populations of Japanese larch

日本落叶松自然种群线粒体DNA变异

• 发表时间: 2009
• 期刊: Larix kaempferi 58
• 作者: San Jose-Maldia;L.;Uchida;K.;Tomaru;N.
• 通讯作者: N.