Phylogeographical study of dipterocarp species and development of molecular database for identification of its origin.

龙脑香属物种的系统发育地理学研究及其起源鉴定分子数据库的开发。

• 批准号: 18255010
• 负责人: TSUMURA Yoshihiko
• 金额: $ 26.96万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18255010/
• 关键词: フタバガキ科; 遺伝構造; 産地識別; 系統地理; 遺伝的多様性

We have collected 920 individuals of 28 populations in Shorea leprosula throughout its distribution area such as Malaysia and Indonesia. In S.parvifolia, 404 individuals of 13 populations were collected from Malaysia and Indonesia. DNA markers for population samples were developed which are 40 loci of EST-SSR markers derived from cDNA sequence data. Intraspecific chloroplast DNA polymorphisms were screened of 17 regions of intergenic regions, and found 5 regions (trnD-trnT、petB intron、petL-psbE、psbJ-petA、rps16-trnK) were polymorphic in S.leprosula and 4 regions petL-psbE、psbJ-petA、rpl32-trnL、trnQ-rps16) in S.parvifolia. The result of nuclear DNA markers such as 34 loci of EST-SSRs showed clear genetic differentiation between Borneo and the other populations, although the Fst=0.0671 was low. The result of chloroplast DNA (cpDNA) polymorphism showed much clear genetic differentiation between Borneo and the other populations, and 21 cpDNA haplotypes was found and the coefficient of genetic differentiation, Fst value was quite high, 0.5851. These results suggest that there were two different origins of current tropical forest in Southeast Asia. According to cpDNA results, region identification was possible but the identification of each population would be difficult by only DNA data.

我们在马来西亚、印度尼西亚等分布地区收集了麻风Shorea 28个种群920只个体。在马来西亚和印度尼西亚共采集到13个种群404只。利用cDNA序列数据开发了40个EST-SSR标记。筛选了17个基因间区叶绿体DNA的种内多态性，发现麻风中有5个区（trnD-trnT、petB内含子、petL-psbE、psbJ-petA、rps16-trnK）存在多态性，细叶中有4个区（petL-psbE、psbJ-petA、rpl32-trnL、trnQ-rps16）存在多态性。34个EST-SSRs位点的核DNA标记结果表明，婆罗洲与其他种群之间存在明显的遗传分化，但Fst=0.0671较低。叶绿体DNA （cpDNA）多态性结果表明，婆罗洲与其他居群之间存在明显的遗传分化，共发现21个cpDNA单倍型，遗传分化系数Fst值较高，为0.5851。这些结果表明，目前东南亚热带森林有两种不同的起源。根据cpDNA结果，可以进行区域鉴定，但仅凭DNA数据难以对每个群体进行鉴定。

项目成果

違法伐採抑止のためのフタバガキ科Shorea属種識別DNAデータベースの構築.

构建龙脑香科望天树属 DNA 数据库，进行物种鉴定，防止非法采伐。

• 发表时间: 2007
• 作者: Wash;M;原田 光;服部 大輔;二宮 生夫;原田 光;津村 義彦
• 通讯作者: 津村 義彦

Effects of flowering tree-density on mat ing system and gene flow in Shorea lepr osula (Dipterocarpaceae) in Peninsular Malaysia.

开花树密度对马来西亚半岛龙脑香科植物交配系统和基因流的影响。

• 发表时间: 2007
• 期刊: Journal of Plant Research 120
• 作者: Fukue;Y.;T. Kado;S.L. Lee;K.K.S. Ng;N. Muhammad;Y. Tsumura
• 通讯作者: Y. Tsumura

Effects of ffowehng tree-density on mating system and gene flow in Shorea leprosula(Dipterocarpaceae)in Peninsular Malaysia

树木密度对马来西亚半岛龙脑香科植物交配系统和基因流的影响

• 发表时间: 2007
• 期刊: Journal of Plant Research 120
• 作者: Fukue;Y.;T. Kado;S. L. Lee;K. K.S. Ng;N. Muhammad;Y. Tsumura
• 通讯作者: Y. Tsumura

ST-SSRs isolated from Shorea leprosula and their transferability to 36 species within the Dipterocarpaceae

从麻风望天树中分离出的 ST-SSR 及其向龙脑香科 36 个物种的转移性

• 发表时间: 2009
• 期刊: Molecular Ecology Resources 9
• 作者: Ng;K. K. S.;S. L. Lee;Y. Tsumura;S. Ueno;C. H. Ng;C. T. Lee
• 通讯作者: C. T. Lee

マイクロサテライトマーカーを用いたフタバガキ科Shorea maxwellianaの遺伝子流動

使用微卫星标记研究望天树的基因流

• 发表时间: 2009
• 作者: Koretsune S.;Fukuda K.;Zhaoyamg C.;Fuchen S.;Ishida A.;増田真佑・谷尚樹・上野真義・Norwati Muhammad・Soon Leong Lee・津村義彦
• 通讯作者: 増田真佑・谷尚樹・上野真義・Norwati Muhammad・Soon Leong Lee・津村義彦