DEVELOPMENT OF MICROCARRIER FOR INTRODUCING LARGE DNA EFFICIENTLY AND MOLECULAR CYTOLOGOCAL ANALYSIS OF ITS INTRODUCED REAGION

点击翻译按钮获取中文摘要

We developed a novel system that introduced large DNA fragments of wheat including the gene cluster related to grain hardness into rice by the use of bioactive beads-mediated transformation. This transformation method does not require the special promoter and the construction of binary vectors. The fragments of wheat genome DNA were stably transmitted to offspring and the transgenes were normally expressed in transgenic rice. The location of large DNA fragments of wheat and the transgenes in rice genome were made visible by FISH and fiber FISH methods.