Chromosome painting and synteny analysis in cereals by multicolor FISH using bacterial artificial chromosomes

使用细菌人工染色体通过多色 FISH 对谷物进行染色体涂色和同线性分析

基本信息

  • 批准号:
    09490024
  • 负责人:
  • 金额:
    $ 6.66万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1999
  • 项目状态:
    已结题

项目摘要

1.Chromosome painting was carried out on chromosomes of cereals using the probes in which rice or Aegilops squarrosa bacterial artificial chromosome(BAC) clones were pooled for each chromosome group. We succeeded in developing a probe labeling-detection system which discriminate each probe with different color by combining three fluorochromes. The seven pooled probe sets were visualized simultaneously in a single FISH experiment. Using this technique, genome donor species to polyploid species were identified in useful plants such as, finger millet, peanut and coffee.2.We have selected 500 clones (including agronomically important genes) from an Ae squarrosa BAC library and applied multicolor FISH to mitotic metaphase chromosomes of Ae. squarrosa and common wheat. Most genes were mapped on the distal ends of chromosomes. The synteny analysis was conducted by comparing the order of FISH signals. Synteny of some genes was conserved beyond plant family.3.We have achieved the direct visualization of gene organization on extended nuclear DNA fibers using FISH in agronomically important genes of cereals. We demonstrated the repetitive nature of the secalin-1 gene cluster of rye, which consists of up to 15 genes arranged in tandem repeats. We also developed high-resolution FISH on BAC DNAs by molecular combing. The fiber FISH technique contributes to construction of BAC contig.4.We have isolated 20 clones containing centromeric repeated sequences from a BAC library of Ae. squarrosa and characterized these clones. We found that several kinds of sequences have high homology to the sequences reported previously in rice, sorghum, barley and corn. FISH analysis has revealed that these sequences are mostly conserved in the centromeric regions of cereals. Fine structure of centromeres was visualized by fiber FISH.
1.以水稻和粗山羊草(Aegilopssquarrosa)细菌人工染色体(BAC)克隆为探针,对禾谷类植物染色体进行了染色。我们成功地开发了一种探针标记检测系统,该系统通过组合三种荧光染料来区分不同颜色的探针。在单个FISH实验中同时可视化七个合并的探针组。从粗山羊草BAC文库中筛选出500个克隆(包括农艺学上重要的基因),应用FISH技术对粗山羊草有丝分裂中期染色体进行了分析。squarrosa和普通小麦。大多数基因定位在染色体末端。通过比较FISH信号的顺序进行同线性分析。部分基因的同线性在植物科外是保守的。3.利用FISH技术实现了禾谷类作物重要农艺性状基因在延伸的核DNA纤维上基因组织的直观可视化。我们证明了黑麦secalin-1基因簇的重复性,它由多达15个串联重复排列的基因组成。我们还开发了高分辨率FISH BAC DNA分子梳理。纤维FISH技术有助于BAC contig的构建。4.从Ae. squarrosa和特点这些克隆。我们发现有几种序列与水稻、高粱、大麦和玉米中已报道的序列有很高的同源性。FISH分析表明,这些序列大多是保守的,在谷物的着丝粒区域。纤维FISH显示着丝粒的精细结构。

项目成果

期刊论文数量(42)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Raina, S.N.: "Genomic in situ hybridization identifies the diploid wild progenitors of cultivated (Arachis bypogaea) and related wild (A. monticola) peanut species"Plant Syst. Evol.. 214. 251-262 (1999)
Raina, S.N.:“基因组原位杂交鉴定了栽培(Arachis bypogaea)和相关野生(A. monticola)花生品种的二倍体野生祖先”Plant Syst.
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Raina,S.N.: "Detection of a variable number of 18S-5.8S-26S and 5S ribosomal DNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis speies." Genome. 42(in press). (1999)
Raina,S.N.:“通过荧光原位杂交在二倍体和四倍体花生物种中检测可变数量的 18S-5.8S-26S 和 5S 核糖体 DNA 位点。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Mukai, Y.: "Physical mapping of genes controlling wheat grain quality by fluorescence in situ hybridization"Proc. 9th Int. Wheat Genet. Symp.. 4. 12-16 (1998)
Mukai,Y.:“通过荧光原位杂交对控制小麦籽粒品质的基因进行物理定位”Proc。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
向井 康比己: "有用植物染色体の分子細胞遺伝学的研究"育種学研究. 1. 165-172 (1999)
Yasuhiki Mukai:“有用植物染色体的分子细胞遗传学研究”育种研究。1. 165-172 (1999)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Mukai,Y., Rahman,S., Yamamoto,M., Okamoto,M., Turner,M., Li,Z., Mukai,Y., Abbott,D., Abrahams,S., Kossar-Hashemi,B., Samuels,M., Appels,R. and Morell,M.K.: "Physical mapping of genes controlling wheat grain quality by fluorescence in situ hybridization"Pr
Mukai,Y.、Rahman,S.、Yamamoto,M.、Okamoto,M.、Turner,M.、Li,Z.、Mukai,Y.、Abbott,D.、Abrahams,S.、Kossar-Hashemi,B
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

MUKAI Yasuhiko其他文献

MUKAI Yasuhiko的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('MUKAI Yasuhiko', 18)}}的其他基金

Identification of genome and chromosomes triggering chromosome elimination in haploid breeding using Imperata cylindrica system
使用白茅系统鉴定单倍体育种中触发染色体消除的基因组和染色体
  • 批准号:
    22580004
  • 财政年份:
    2010
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF MICROCARRIER FOR INTRODUCING LARGE DNA EFFICIENTLY AND MOLECULAR CYTOLOGOCAL ANALYSIS OF ITS INTRODUCED REAGION
高效导入大DNA微载体的开发及其导入区域的分子细胞学分析
  • 批准号:
    19380194
  • 财政年份:
    2007
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了