A Integrated Study of Pathological Significance of Cytosine Methylation of Non-CpG Island

非CpG岛胞嘧啶甲基化病理意义的综合研究

• 批准号: 19390100
• 负责人: KITAZAWA Sohei
• 金额: $ 6.49万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390100/
• 关键词: 非CpCアイランド; メチル化; 遺伝子プロモータ; 非CpGアイランド; 遺伝プロモータ; 膀胱癌; 造血細胞; 転写因子; エピジェネティクス; CpG-island; DNAメチル化; BANBI; 転写制御; 副甲状腺過形成; P16; BAMBI

Cytosine methylation in CpG-island, located usually regulatory region of many genes, is well recognized as an inhibitory epigenetic regulator. On the other hand, biological significance of the cytosine methylation other than CpG-island is unclear. By focusing morphology-oriented microscopic dissection methods, we analyzed cytosine methylation status of the BAMBI gene promoter in bladder cancer and of the P16 in renal tumor of diabetic rats, and found that some of the cytosine methylation outside of the CpG-island contributes to the tumor development and progression.

CpG岛是许多基因的调控区域，其胞嘧啶甲基化被认为是一种抑制性表观遗传调控因子。另一方面，除CpG岛外，胞嘧啶甲基化的生物学意义尚不清楚。通过聚焦形态学显微解剖方法，分析了膀胱癌中BAMBI基因启动子和糖尿病大鼠肾肿瘤中P16基因启动子的胞嘧啶甲基化状态，发现CpG岛外的部分胞嘧啶甲基化参与了肿瘤的发生和发展。

项目成果

Hypermethylation of epithelial-cadherin gene promoter is associated with Epstein-Barr virus in nasopharyngeal carcinoma

• DOI: 10.1016/j.cdp.2008.05.005
• 发表时间: 2008-01-01
• 期刊: CANCER DETECTION AND PREVENTION
• 作者: Niemhom, Sopaporn;Kitazawa, Sohei;Leopairat, Juvady
• 通讯作者: Leopairat, Juvady

Modulation of Mouse RANKL Gene Expression by Runx2 and Vitamin D3

• DOI: 10.1002/jcb.21929
• 发表时间: 2008-12-01
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Kitazawa, Riko;Mori, Kiyoshi;Kitazawa, Sohei
• 通讯作者: Kitazawa, Sohei

MeCP2 expression and promoter methylation of cyclin D1 gene are associated with cyclin D1 expression in developing rat epididymal duct.

Cyclin D1基因的MECP2表达和启动子甲基化与Cyclin d1表达在发育中的大鼠附子导管中有关。

• DOI: 10.1267/ahc.08025
• 发表时间: 2008-10-29
• 期刊: ACTA HISTOCHEMICA ET CYTOCHEMICA
• 影响因子: 2.4
• 作者: Darwanto, Agus;Kitazawa, Riko;Mori, Kiyoshi;Kondo, Takeshi;Kitazawa, Sohei
• 通讯作者: Kitazawa, Sohei

ラット酸化的ストレスモデルにおける腎腫瘍発生初期に生じるエピジェネティクス変化の解析

大鼠氧化应激模型中肾肿瘤发展早期发生的表观遗传变化分析

• 发表时间: 2010
• 作者: 北澤荘平;他
• 通讯作者: 他

FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets

• DOI: 10.1172/jci34090
• 发表时间: 2008-08-01
• 期刊: JOURNAL OF CLINICAL INVESTIGATION
• 影响因子: 15.9
• 作者: Nishino, Naonobu;Tamori, Yoshikazu;Kasuga, Masato
• 通讯作者: Kasuga, Masato