Molecular mechanism of accumulation and rearrangement of antibiotic resistance gene in bacteria.

细菌抗生素抗性基因积累和重排的分子机制。

• 批准号: 19390127
• 负责人: ARAKAWA Yoshichika
• 金额: $ 11.07万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390127/
• 关键词: 薬剤耐性菌; 遺伝子転位機構; クラス3インテグロン; アシネトバクター; OXA型β-ラクタマーゼ; カルバペネム耐性; 耐性遺伝子; インテグラーゼ; 再配列; Serratia marcescens; Klebsiella pneumoniae; IntI3; IMP-1; AAC(6')-Ib; VRE; vanA

We determined the genetic structure of class 3 integrons in Serratia marcescens and Pseudomonas putida clinical isolates. The nucleotide regions including class 3 integron were flanked by 25bp inverted repeats and co-located with the elements such as TniA, TniB, TniQ, and TniC, which are thought to be required for the transposition of class 3 integron. Recombinant IntI3 protein was expressed in Escherichia coli, purified using column chromatography, and crystallized by hanging drop vapor diffusion method. However, we could not obtain a good X-ray diffraction data from the crystals. In Acinetobacter baumannii clinical isolates, ISAbaI was located upstream of bla_<OXA-51-like>, and apperared to provide a promoter activity for expression of adjacent bla_<OXA-51-like>.

我们测定了粘质沙雷菌和恶臭假单胞菌临床分离株3类整合子的遗传结构。包括第3类整合子在内的核苷酸区域两侧有25bp的反向重复序列，并与TniA、TniB、TniQ和TniC等元件共定位，这些元件被认为是第3类整合子转位所必需的。重组IntI3蛋白在大肠杆菌中表达，柱层析纯化，悬垂滴气扩散法结晶。然而，我们无法从晶体中获得良好的x射线衍射数据。在鲍曼不动杆菌临床分离株中，ISAbaI位于bla_<OXA-51-like>的上游，似乎为邻近的bla_<OXA-51-like>的表达提供了启动子活性。