Genetic Analyzes of Biosynthesis Mechanism of Capsular Polysaccharide as a Pathogenic Determinant.

荚膜多糖作为致病决定因素的生物合成机制的遗传分析。

• 批准号: 06670288
• 负责人: ARAKAWA Yoshichika
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670288/
• 关键词: Klebsiella pneumoniae; capsular polysaccharide; Gene organization; Klebsiella pneumoniae; クレブシエラ; 多糖合成遺伝子; 糖転移酵素; 病原因子; 塩基配列; ポリシストロン

In these two years, we have been studying on the biosynthesis mechanisms of bacterial capsular polysaccharide (CPS) which has been regarded as one of the bacterial pathogenic determinants. The cps gene cluster of K pneumoniae Chedid (K2 : O1), which is involved in the biosynthesis of serotype K2 CPS,has been subcloned into M13 phages, mp 18 and mp 19, and the entire nucleotide sequence, about 24-kb, was determined in the term of this project. This investigation provided us many important new findings and information about the structure of the Klebsiella cps cluster and its regulation. At least 19 putative open reading frames (ORFs) were identified in the sequenced area, and several genes similar to the cpsG,cpsB,gnd, and galF were found in this region. The region implicated in the synthesis of serotype K2 CPS was localized in the central portion of the sequenced area, and it was suggested that the expression of genes in this region still continue even in the stationary phase by the function of a gene locating in the upstream region of the ORF3. However, the functions of several genes are still unknown, therefore we are planning to continue the analyzes of their roles in the biosynthesis of CPS in the next term of investigation.

近两年来，我们一直在研究细菌荚膜多糖（CPS）的生物合成机制，CPS被认为是细菌致病的决定因素之一。本课题将参与K_2CPS生物合成的肺炎克雷伯菌Chedid（K_2：O_1）的cps基因簇亚克隆到M_13、mp_（18）和mp_（19）中，并测定了其全长约24kb的核苷酸序列。本研究为克雷伯氏菌cps簇的结构及其调控提供了许多重要的新发现和信息。在测序区域至少鉴定出19个开放阅读框架（ORF），并发现了几个与cpsG、cpsB、gnd和galF相似的基因。与血清型K2 CPS的合成有关的区域位于测序区域的中心部分，并且表明该区域中的基因的表达通过位于ORF 3的上游区域中的基因的功能即使在稳定期仍然继续。然而，一些基因的功能仍然是未知的，因此，我们计划在下一个研究阶段继续分析它们在CPS生物合成中的作用。

项目成果

Yoshichika Arakawa et al.: "Molecular Approaches to Food Safety.Edited by M.Eklund et al.Genetic Analyses of Klebsiella cps Gene Cluster…" Alaken,Inc.Fort Collins,Colorado., 30 (1995)

Yoshichika Arakawa 等人：“食品安全的分子方法。由 M.Eklund 等人编辑。克雷伯菌 cps 基因簇的遗传分析……”Alaken,Inc.Fort Collins,Colorado., 30 (1995)

Yoshichika Arakawa et al.: "Molecular Approach to Food Safety.Edited by M.Eklund et al.Genetic Analyses of Klebsiella cps Gene Cluster" Alaken,Inc.Fort Collins,Colorado,USA, 30 (1995)

Yoshichika Arakawa 等人：“食品安全的分子方法。M.Eklund 等人编辑。克雷伯菌 cps 基因簇的遗传分析”Alaken,Inc.Fort Collins,Colorado,USA, 30 (1995)

荒川宜親: "Klebsiella pnenmoniaeの病原因子としての莢膜多糖体合成に関与する遺伝子領域cpsの構造と発現調節" 日本細菌学雑誌. 49. 455-463 (1994)

Yoshichika Arakawa：“cps 的结构和表达调控，cps 是肺炎克雷伯氏菌致病因子中参与荚膜多糖合成的基因区域”，《日本细菌学杂志》49. 455-463 (1994)。

Yoshichika Arakawa et al.: "Genomic Organization of the Klebsiella pneumoniae cps Region Responsible for Serotype K2 Capsular Polysaccharide Synthesis in the Virulent Strain Chedid." J.Bacteriology. 177. 1-1796 (1995)

Yoshichika Arakawa 等人：“肺炎克雷伯菌 cps 区域的基因组组织负责强毒菌株 Chedid 中血清型 K2 荚膜多糖的合成。”

荒川宜親: "Klebsiella pneumoniaeの病原因子としての莢膜多糖体合成に関与する遺伝子領域cpsの構造と発現調節" 日本細菌学雑誌. 49. 455-463 (1994)

Yoshichika Arakawa：“作为肺炎克雷伯菌的致病因子参与荚膜多糖合成的基因区域 CPS 的结构和表达调节”日本细菌学杂志 49. 455-463 (1994)。