Molecular mechanisms on differentiation or function of osteoblasts and osteoclasts by the Wnt/LRP signaling pathway

Wnt/LRP信号通路影响成骨细胞和破骨细胞分化或功能的分子机制

• 批准号: 19390469
• 负责人: TAMURA Masato
• 金额: $ 12.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390469/
• 关键词: 骨形成; 骨吸収; Wntシグナル; 骨芽細胞; Wnt; BMP; オステオプロテゲリン; miRNA

In this study, we demonstrated that two cellular signaling pathways of Wnt and bone morphogenetic protein (BMP) control both differentiation and function of osteoblasts and osteoclasts during processes of bone formation and resorption. These Wnt and BMP signaling regulates osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor, which can inhibit the production of osteoclasts. The regulation of OPG expression is mediated through two transcription pathways such as Wnt and BMP that involve the OPG promoter. We also demonstrated that osteoblastic differentiation mediated by certain miRNA expression and BMP-2 down-regulates miR-206 expression at the post-transcriptional level and that BMP-2 could regulate miRNA biogenesis by a novel mechanism.

在这项研究中，我们证明了Wnt和骨形态发生蛋白（BMP）的两个细胞信号通路控制成骨细胞和破骨细胞在骨形成和吸收过程中的分化和功能。这些Wnt和BMP信号调节骨保护素（OPG），也称为破骨细胞生成抑制因子，其可以抑制破骨细胞的产生。OPG表达的调控通过两条转录途径介导，如Wnt和BMP，它们涉及OPG启动子。我们还证明了由某些miRNA表达介导的成骨细胞分化和BMP-2在转录后水平下调miR-206表达，并且BMP-2可以通过新的机制调节miRNA生物合成。

项目成果

Wnt/β-cateninシグナルによって誘導される遺伝子のosteoprotegerin発現調節機構の解析

Wnt/β-catenin信号诱导的骨保护素基因表达调控机制分析

• 发表时间: 2008
• 作者: 佐藤真理;田村正人;八若保孝
• 通讯作者: 八若保孝

Wnt signaling inhibits cementoblast differentiation and promotes proliferation

• DOI: 10.1016/j.bone.2008.12.029
• 发表时间: 2009-05-01
• 期刊: BONE
• 影响因子: 4.1
• 作者: Nemoto, Eiji;Koshikawa, Yohei;Shimauchi, Hidetoshi
• 通讯作者: Shimauchi, Hidetoshi

Enamel matrix derivative neutralized the effect of lipopolysaccharide on osteoprotegerin and receptor activator of nuclear factor kappa B ligand expression of osteoblasts

牙釉质基质衍生物中和脂多糖对成骨细胞护骨素和核因子κB受体激活剂配体表达的影响

• 发表时间: 2009
• 期刊: Arch Oral Biol 54(4)
• 作者: Wada Y;Mizuno M;Tamura M
• 通讯作者: Tamura M

The suppressive effect of enamel matrix derivative on osteocalcin gene expression of osteoblast is neutralized by an antibody against TGF-s

釉质基质衍生物对成骨细胞骨钙素基因表达的抑制作用被 TGF-s 抗体中和

• 发表时间: 2008
• 期刊: Periodont 79(2)
• 作者: Wada Y;Mizuno M;Yamamoto E;Nambu S;Tamura;M
• 通讯作者: M

Regulation of microRNA expression by bone morphogene tic protein-2(T. Sasano, O. Suzuki (Eds.))

骨形态发生蛋白-2对微小RNA表达的调节（T. Sasano，O. Suzuki（Eds.））

• 发表时间: 2010
• 作者: Sato M;Yawaka Y;Tamura M
• 通讯作者: Tamura M