Development of novel photo cross linkable groups for explorative study of target protein

开发新型光交联基团用于目标蛋白的探索性研究

• 批准号: 19510213
• 负责人: HOSOYA Takamitsu
• 金额: $ 2.91万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19510213/
• 关键词: 化学修飾; タンパク質; 光反応; 光親和性標識; ジアジリン; アジド; アセチレン; 非放射性; 光親和性標識法; Click反応

以前独自に開発した、bioorthogonalな官能基であるアジド基やエチニル基の特性を活用した非放射性光親和性標識法のさらなる有効化を目指し、光反応性官能基であるジアジリニル基とアジド基またはエチニル基とを融合させた新規検出基導入用タグ一体型の光クロスリンク官能基の開発を試みた。その結果、アジドジフルオロメチルジアジリニル基およびエチニルジフルオロメチルジアジリニル基を有する化合物の合成に成功するとともに、光反応および修飾反応のモデル実験からこれら官能基の実用性を示すことができた。

In the past, we used to use the non-radioactive light and the properties of the bioorthogonal function-based communication system independently. In the past, we used to use the non-radioactive light and the performance analysis method to determine the performance of the system. In the past, we used to do this alone, and we used to use the non-radioactive light-based method and the property-based method. The results showed that the compounds were successfully synthesized, and the results showed that the compounds were successfully synthesized. the results showed that the compounds were successfully synthesized. the results showed that the compounds were successfully synthesized. the results showed that the compounds were successfully synthesized. the results showed that the compounds were successfully synthesized. the results showed that the functional basis was not available.

项目成果

Facile synthesis of diazido functionalized biaryl compounds as radioisotope free photoaffinity probes by Suzuki Miyaura coupling

通过 Suzuki Miyaura 偶联轻松合成二叠氮官能化联芳基化合物作为无放射性同位素光亲和探针

• 发表时间: 2009
• 期刊: Bioorg. Med. Chem 17(6)
• 作者: T. Hosoya;A. Inoue;T. Hiramatsu;H. Aoyama;T. Ikemoto;and M. Suzuki
• 通讯作者: and M. Suzuki

Novel method of radioisotope-free photoaffinity labeling using bioorthogonal group as a detectable tag function

使用生物正交群作为可检测标签函数的无放射性同位素光亲和标记的新方法

• 发表时间: 2008
• 作者: Takamitsu Hosoya;ToshiyukiHiramatsu;Atsushi Inoue;YingGuo;Isao Kii;Akira Kudo
• 通讯作者: Akira Kudo

Facile synthesis of diazido-functionalized biaryl compounds as radioisotope-free photoaffinity probes by Suzuki-Miyaura coupling.

通过 Suzuki-Miyaura 偶联轻松合成二叠氮基官能化联芳基化合物作为无放射性同位素光亲和探针。

• 发表时间: 2009
• 期刊: Bioorg. Med. Chem. 17
• 作者: T. Hosoya;A. Inoue;T. Hiramatsu;H. Aoyama;T. Ikemoto;M. Suzuki
• 通讯作者: M. Suzuki

Novel method of radioisotope free photoaffinity labeling using bioorthogonal group as a detectable tag function

使用生物正交群作为可检测标签函数的无放射性同位素光亲和标记的新方法

• 发表时间: 2008
• 作者: Takamitsu Hosoya;Toshiyuki Hiramatsu;Atsushi Inoue;Ying Guo;Isao Kii;Akira Kudo
• 通讯作者: Akira Kudo

ジアジド型non-RI光親和性標識プローブの簡便合成法の開発

开发二叠氮型非 RI 光亲和标记探针的简单合成方法

• 发表时间: 2008
• 作者: 井上敦;平松俊行;鈴木正昭;細谷孝充
• 通讯作者: 細谷孝充