Development of new coelenterazine analogue for in vivo imaging of reactive oxygen species

活性氧不仅作为细胞内和细胞间的信使，而且还参与炎症或癌症等疾病的发病。在这项研究中，我们的目的是发展一种有效的方法，可以定量检测活性氧在真实的时间与高灵敏度。由于硼对某种活性氧具有很高的亲和力，我们发展了一种新的合成含硼有机化合物的方法。我们还合成了一种新的腔肠素类似物，作为腔肠素型生物发光系统的有效底物。

Reactive oxygen species are not only used as an intra- and intercellular messenger, but also involved in the onset of diseases such as inflammation or cancer. In this study, we aimed to develop an efficient method that can quantitatively detect reactive oxygen species in real time with high sensitivity. Since boron shows high affinity toward a certain type of reactive oxygen species, we developed a new synthetic method of organoboron-containing componds. We also synthesized a new coelenterazine analogue which served as an efficient substrate for a coelenterazine-type bioluminescence system.