Development of new coelenterazine analogue for in vivo imaging of reactive oxygen species

活性氧不仅被用作细胞内和细胞间的信使，而且还参与诸如炎症或癌症等疾病的发生。在本研究中，我们旨在开发一种能够实时、高灵敏度地定量检测活性氧的有效方法。由于硼对某种类型的活性氧具有高亲和力，我们开发了一种新的合成有机含硼化合物的方法。我们还合成了一种新的coelenterazine类似物，作为coelenterazine型生物发光系统的有效底物。

Reactive oxygen species are not only used as an intra- and intercellular messenger, but also involved in the onset of diseases such as inflammation or cancer. In this study, we aimed to develop an efficient method that can quantitatively detect reactive oxygen species in real time with high sensitivity. Since boron shows high affinity toward a certain type of reactive oxygen species, we developed a new synthetic method of organoboron-containing componds. We also synthesized a new coelenterazine analogue which served as an efficient substrate for a coelenterazine-type bioluminescence system.