Development of a new culture process to prevent an oncogenic transformation of the stem cells

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The aim of this research is to develop a new culture process to prevent an oncogenic transformation of hepatic stem cells for their safety application to regenerative medicine. First, we established three estimation methods for the oncogenic transformation of the stem cells. Second, we found that the porous hydroxyapatite culture carrier was suitable for mass culture of the stem cells, and the pore size and raggedness of the surface were important to prevent the oncogenic transformation and enhance the hepatic activity of the stem cells. Third, we succeeded in the selective elimination of transformed cells in the cultivated stem cells by using hybrid liposomes, which originally developed in our research.