Development of genetic system of rRNA genes and its use in B. subtilis

rRNA基因遗传系统的开发及其在枯草芽孢杆菌中的应用

• 批准号: 19570006
• 负责人: KAWAMURA Fujio
• 金额: $ 2.83万
• 依托单位: Rikkyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19570006/
• 关键词: リボソーム; rRNA; 翻訳; 枯草菌; 胞子形成; rRNAオペロン; 抗生物質耐性

We deleted the 10 rrn operons of B. subtilis sequentially, and constructed seven mutants, each harboring a single rrn operon in their genome. The growth rates and sporulation frequencies of these mutants were reduced drastically as compared with those of the wild type strain, indicating that normal growth as well as sporulation requires multiple copy numbers of rRNA operon. Further analysis using mutants having various numbers of rRNA operon showed that normal sporulation requies more than 5 copies of rRNA operon.

我们依次删除了枯草杆菌的10个rrn操纵子，并构建了7个突变体，每个突变体的基因组中都含有一个rrn操纵子。与野生型菌株相比，这些突变体的生长速度和产孢率都显著降低，表明正常生长和产孢量都需要多拷贝的rRNA操纵子。用具有不同数量的rRNA操纵子的突变体进一步分析表明，正常的孢子形成需要超过5个拷贝的rRNA操纵子。

项目成果

Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis

• DOI: 10.1111/j.1365-2958.2007.06018.x
• 发表时间: 2008-01-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Nanamiya, Hideaki;Kasai, Koji;Tozawa, Yuzuru
• 通讯作者: Tozawa, Yuzuru

A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis

• DOI: 10.1111/j.1365-2958.2006.05513.x
• 发表时间: 2007-01-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Natori, Yousuke;Nanamiya, Hideaki;Kawamura, Fujio
• 通讯作者: Kawamura, Fujio

Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillua subtilis rsmG mutants.

鉴定 RsmG 甲基转移酶靶标为 16S rRNA 核苷酸 G527，并表征枯草芽孢杆菌 rsmG 突变体。

• 发表时间: 2007
• 期刊: J. Bacteriol. 189
• 作者: Nishimura K.;Johansen S.K.;Inaoka T.;Hosaka T.;Tokuyama S.;Tahara Y.;Okamaoto S.;Kawamura F.;Douthwaite S.;Ochi K.
• 通讯作者: Ochi K.

Isolation and characterization of sporulation-initiation mutation in theBaeillus subtilis prfB gene

枯草芽孢杆菌 prfB 基因孢子形成起始突变的分离和表征

• 发表时间: 2007
• 期刊: Biosci.Biotechnol.Biochem 71
• 作者: Tsuchimoto;S.;Hirao;Y.;Ohtsubo;E.;and Ohtsubo;H.;Asai Kei
• 通讯作者: Asai Kei

Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene. Biosci.

枯草芽孢杆菌 prfB 基因中孢子形成起始突变的分离和表征。

• 发表时间: 2007
• 期刊: Biotechnol. Biochem. 71
• 作者: Asai;K.;Inaoka;T.;Nanamiya;H.;Sadaie;Y.;Ochi;K.;Kawamura;F.
• 通讯作者: F.