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Reconstitution of protein synthesis system of mammals

Reconstitution of protein synthesis system of mammals
哺乳动物蛋白质合成系统的重建
批准号:
19580113
负责人:
IMATAKA Hiroaki
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009

项目摘要

项目成果

IMATAKA Hiroaki的其他基金

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中文摘要
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英文摘要
We purified mammalian translation initiation factors : eIF1, eIF1A, eIF2, eIF2B, eIF3, eIF4E, eIF4A, eIF4B, eIF4G, eIF5, eIF5B, elongation factors : eEF1A eEF1B・eEF2, termination factors : eRF1、eRF3, aminoacyl tRNA, and ribosome subunits (40S, 60S). By mixing all these factors, we successfully established a reconstituted protein synthesis system derived from mammals.
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会议论文
Requirement of RNA-binding of Mammalian eIF4GI for Efficient Interaction of eIF4E with mRNA Cap
哺乳动物 eIF4GI 的 RNA 结合对于 eIF4E 与 mRNA 帽有效相互作用的要求
DOI: --
发表时间: 2009
期刊: Mol.Cell.Biol. 29
影响因子: --
作者: [Satoshi Mikami, Tominari Kobayashi, Kodai Machida, Mamiko Masutani, Shigeyuki Yokoyama, Hiroaki Imataka, Y.Svitkin, Michiko Kono, Michiko Kono, A.Yanagiya]
通讯作者: A.Yanagiya
DOI: 10.1016/j.pep.2008.09.002
发表时间: 2008-12-01
期刊: PROTEIN EXPRESSION AND PURIFICATION
影响因子: 1.6
作者: [Mikami, Satoshi, Kobayashi, Tominari, Imataka, Hiroaki]
通讯作者: Imataka, Hiroaki
DOI: 10.1016/j.jmb.2009.07.054
发表时间: 2009-10-02
期刊: JOURNAL OF MOLECULAR BIOLOGY
影响因子: 5.6
作者: [Hiyama, Takuya B., Ito, Takuhiro, Yokoyama, Shigeyuki]
通讯作者: Yokoyama, Shigeyuki
A novel human in vitro translation system for glycoprotein expression
一种用于糖蛋白表达的新型人类体外翻译系统
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [K. Vattem, S. Mikami, K. Maas, E. Hommema, A. Morgan, M. Schofield, B. Webb, A. Deshpande, H. Imataka]
通讯作者: H. Imataka
26
    In vitro-synthesis of highly pure viral particles and their application to nanotechnology
    • 批准号:
      22560774
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.83万
    • 财政年份:
      2010
    • 负责人:
      IMATAKA Hiroaki
    • 依托单位:
    Development of the complete translation system reconstituted with human factors toward the synthesis of artificial viruses
    • 批准号:
      20200056
    • 项目类别:
      Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
    • 资助金额:
      $19.47万
    • 财政年份:
      2008
    • 负责人:
      IMATAKA Hiroaki
    • 依托单位: