In vitro-synthesis of highly pure viral particles and their application to nanotechnology

点击翻译按钮获取中文摘要

We established procedures through which RNA viruses, EMCV and HEV are synthesized in vitro. We synthesized EMCV in the human cell extract-derived in vitro protein expression system from DNA, and purified it for analysis with a transmission electron microscope. HEV capsid proteins were produced in E. coli and purified. The purified HEV proteins were incubated in vitro for the formation of HEV viral capsules. To apply these viral particles to drug delivery and bio-imaging, the amino acid sequences of the capsid proteins of EMCV and HEV were altered.