Molecular mechanisms for dissolution of water-insoluble minerals containing phosphates by mycorrhizal fungi

菌根真菌溶解含磷酸盐的水不溶性矿物质的分子机制

• 批准号: 19580172
• 负责人: HATTORI Takefumi
• 金额: $ 3.08万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580172/
• 关键词: 森林生物; Laccaria bicolor; シュウ酸; クエン酸; リンゴ; リン; 酸性土壌; 外生菌根菌; 荒廃地造林; リンゴ酸; Fomitopsis palustris; オキサロ酢酸加水分解酵素; グリオキシル酸脱水素酵素

Ectomycorrhizal (ECM) fungi solubilize water-insoluble minerals containing phosphorus, making phosphate available in soluble form to be taken up by the host tree. Strong chelators, such as oxalate, citrate, and malate, released from ECM play a pivotal role in the dissolution of minerals through complexolysis. In this study, we attempted to clone a cDNA encoding a homologue of the oxalate-producing enzyme cytochrome c dependent glyoxylate dehydrogenase from Laccaria bicolor. We isolated cDNA encoding the cytochrome c dependent glyoxylate dehydrogenase (FpGLOXDH) from Fomitopsis palustris. We found that the homologue of FpGLOXDH (jgi|Lacbil|296363|er2 Lbscfoolog 01720, Laccaria bicolor) was present in the genome of L.bicolor. However, activity of FpGLOXDH was not detected in L.bicolor. Further research is needed to elucidate the mechanism underlying the biosynthesis of these organic acids.

外生菌根（ECM）真菌溶解水不溶性的含磷矿物质，使磷酸盐以可溶形式被宿主树木吸收。从ECM释放的强螯合剂，如草酸盐、柠檬酸盐和苹果酸盐，通过络合溶解在矿物质中起关键作用。在这项研究中，我们试图克隆一个cDNA编码的同源物的脂肪酸产生酶细胞色素c依赖乙醛酸脱氢酶从双色蜡蘑。我们从沼泽拟层孔菌中分离了编码细胞色素c依赖的乙醛酸脱氢酶（FpGLOXDH）的cDNA。我们发现FpGLOXDH（jgi）的同源物|拉克比尔|296363| er2 Lbscfoolog 01720，双色蜡蚧（Laccaria bicolor）基因组中存在。然而，FpGLOXDH的活性在双色乳杆菌中未检测到。需要进一步的研究来阐明这些有机酸的生物合成机制。

项目成果

Subcellular localization of the oxalic acid-producing enzyme, cytochrome c dependent glyoxylate dehydrogenase, in brown-rot fungus Fomitopsis palustris

褐腐真菌 Fomitopsis palustris 中产生草酸的酶（细胞色素 C 依赖性乙醛酸脱氢酶）的亚细胞定位

• 发表时间: 2007
• 期刊: Cellulose Chemistry and Technology 51
• 作者: Hattori;T.;Okawa;K.;Fujimura;M.;Mizoguchi;M.;Watanabe;T.;Tokimatsu;T.;Inui;H.;Baba;K.;Suzuki S.;Umezawa;T.;Shimada;M.
• 通讯作者: M.

アルミニウムによる外生菌根菌の有機酸代謝変動

铝诱导外生菌根真菌有机酸代谢的变化

• 发表时间: 2010
• 作者: 小篠貴臣;他6名
• 通讯作者: 他6名