Molecular mechanisms for dissolution of water-insoluble minerals containing phosphates by mycorrhizal fungi

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Ectomycorrhizal (ECM) fungi solubilize water-insoluble minerals containing phosphorus, making phosphate available in soluble form to be taken up by the host tree. Strong chelators, such as oxalate, citrate, and malate, released from ECM play a pivotal role in the dissolution of minerals through complexolysis. In this study, we attempted to clone a cDNA encoding a homologue of the oxalate-producing enzyme cytochrome c dependent glyoxylate dehydrogenase from Laccaria bicolor. We isolated cDNA encoding the cytochrome c dependent glyoxylate dehydrogenase (FpGLOXDH) from Fomitopsis palustris. We found that the homologue of FpGLOXDH (jgi|Lacbil|296363|er2 Lbscfoolog 01720, Laccaria bicolor) was present in the genome of L.bicolor. However, activity of FpGLOXDH was not detected in L.bicolor. Further research is needed to elucidate the mechanism underlying the biosynthesis of these organic acids.