Removal of copper from wood waste treated with copper-containing wood preservative

用耐铜腐木担子菌palustris和Antrodia xanta分别培养硫酸铜处理过的木材。在第2周，当木材的重量没有下降时，真菌分泌的草酸盐在木块表面和真菌菌丝体之间的界面上沉淀草酸铜络合物，molooite。铜的去除率分别为42.9% （F. palustris）和34.7% （A. xantha）。此外，palustris的菌丝体被证明可以将木材标本中的铜运输到远离木块的地方。另一方面，根据编码两种草酸生成酶的基因转录本的数量，对palustris的草酸合成途径进行了评估：草酰乙酰水解酶（FpOAH）和乙醛酸脱氢酶（FpGLOXDH）。FpOAH在该真菌中主要负责草酸盐的生物合成。

Copper-sulfate treated wood was cultivated with copper tolerant wood-rotting basidiomycete Fomitopsis palustris and Antrodia xanta, separately. At 2 weeks, when weight loss of the wood has not been observed, oxalate secreted from fungi precipitated copper oxalate complex, moolooite, on interface between the wood block surface and the fungal mycelia colonized on it. Removal of moolooite together with the colonized mycelia, 42.9% (F. palustris) and 34.7% (A. xantha) of copper was removed. Furthermore, the mycelia of F. palustris were shown to transport copper from wood specimens to a place far from the wood block. On the other hand, an oxalate biosynthetic pathway in F. palustris was assessed based on amounts of gene transcripts encoding two oxalate-producing enzymes: oxaloacetase acetylhydrolase (FpOAH) and glyoxylate dehydrogenase (FpGLOXDH). FpOAH was found to be mainly responsible for oxalate biosynthesis in this fungus.