Removal of copper from wood waste treated with copper-containing wood preservative

用含铜木材防腐剂处理的木材废料中铜的去除

• 批准号: 23580455
• 负责人: HATTORI Takefumi
• 金额: $ 3.33万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580455/
• 关键词: 銅除去; 銅耐性菌; オオウズラタケ; チョークアナタケ; シュウ酸; シュウ酸銅; 銅運搬; 木材防腐剤; 銅の除去; 褐色腐朽菌; Wood-rotting fungi; Fomitopsis palustris; Removal of copper; copper-tolerant fungi; 木材腐朽菌; 銅

Copper-sulfate treated wood was cultivated with copper tolerant wood-rotting basidiomycete Fomitopsis palustris and Antrodia xanta, separately. At 2 weeks, when weight loss of the wood has not been observed, oxalate secreted from fungi precipitated copper oxalate complex, moolooite, on interface between the wood block surface and the fungal mycelia colonized on it. Removal of moolooite together with the colonized mycelia, 42.9% (F. palustris) and 34.7% (A. xantha) of copper was removed. Furthermore, the mycelia of F. palustris were shown to transport copper from wood specimens to a place far from the wood block. On the other hand, an oxalate biosynthetic pathway in F. palustris was assessed based on amounts of gene transcripts encoding two oxalate-producing enzymes: oxaloacetase acetylhydrolase (FpOAH) and glyoxylate dehydrogenase (FpGLOXDH). FpOAH was found to be mainly responsible for oxalate biosynthesis in this fungus.

用耐铜腐木担子菌沼泽拟层孔菌和洋菇分别培养硫酸铜处理的木材。2周后，当木材未发生失重时，真菌分泌的草酸盐在木材表面与真菌菌丝体的界面上沉淀出草酸铜络合物钼铅矿，钼铅矿与菌丝体的去除率为42.9%（F. palustris）和34.7%（A.黄原胶）的铜。此外，F.沼泽地被证明运输铜从木材标本到一个地方远离木块。另一方面，F.基于编码两种产生草酸的酶的基因转录物的量来评估沼泽地芽孢杆菌：草酰乙酸酶乙酰水解酶（FpOAH）和乙醛酸脱氢酶（FpGLOXDH）。发现FpOAH主要负责该真菌中草酸的生物合成。

项目成果

銅耐性褐色腐朽菌オオウズラタケ(Fomitopsis palustris)のシュウ酸生合成におけるオキサロ酢酸加水分解酵素の役割

草酰乙酸水解酶在耐铜褐腐真菌 Fomitopsis palustris 草酸生物合成中的作用

• 发表时间: 2012
• 作者: 久森弘道;渡邉知樹;鈴木史朗;大川久美子;酒井温子;吉村 剛;梅澤俊明;服部武文
• 通讯作者: 服部武文

Cloning and expression analysis of a cDNA encoding an oxaloacetate acetylhydrolase from the brown-rot fungus Fomitopsis palustris

褐腐真菌 Fomitopsis palustris 草酰乙酸乙酰水解酶 cDNA 的克隆和表达分析

• 发表时间: 2013
• 期刊: Sustainable Humanosphere
• 作者: Hiromichi Hisamori;Tomoki Watanabe;Shiro Suzuki;Kumiko Okawa;Haruko Sakai;Tsuyoshi Yoshimura;Toshiaki Umezawa and Takefumi Hattori
• 通讯作者: Toshiaki Umezawa and Takefumi Hattori