Analysis of terpenoid biosynthesis-related enzyme and gene for control of wood extractives

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In order to understand the system and control method for terpenoid metabolism in Cupressu lusitanica cultured cell as a model of woody plant cell, mRNA and enzyme assay were performed. Membrane array method to analyze mRNA activation showed 22 clones were probably for attacking fungi and 1 clone, enolase, would be involved in the supply of isoprenoid precursor with MEP pathway.Crude P450 enzymes obtained from C.lusitanica cell would be involved in terpene oxidation were also characterized. Major stream of terpene oxidation was initiated from terpinolene and have typical oxidation steps, hydroxylation and epoxilation. Interstingly, these reactions were very strictly stereoselective(e.e=ca.100%).