Analysis of the mechanisms by which cyclin D1 controls hexokinase 2 function in breast cancer

乳腺癌中cyclin D1控制己糖激酶2功能的机制分析

• 批准号: 19590087
• 负责人: SAKAMAKI Toshiyuki
• 金额: $ 2.75万
• 依托单位: Nigata University of Phermacy and Applied Life Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590087/
• 关键词: 遺伝子; 癌; 細胞・組織; シグナル伝達; 蛋白質

ヒト乳腺上皮細胞株MCF10AにCyclin D1及びHexokinase 2を過剰発現させた安定発現株の樹立に成功した。また、ヒト乳癌細胞株MDA-MB-453及びSKBR3 にCyclin D1に対するsiRNAに導入し、安定発現株を樹立した。上記の安定発現株を用いた解析の結果、Cyclin D1がDNAメチル基転移酵素の発現に影響を及ぼす可能性が示唆され、さらに、Hexokinase 2を含む複数の遺伝子のプロモーターのメチル化状況の網羅的な解析を行った。

Breast epithelial cell lines MCF10A-Cyclin D1 and Hexokinase-2 were successfully screened for diazepam. MDA-MB-453, breast cancer cell lines MDA-MB-453 and SKBR3, Cyclin D1, siRNA, diazepam and diazepam were isolated. The results of the previous analysis of the diazepam plant, the Cyclin D1 DNA gene transfer enzyme analysis, and the possibility that the Hexokinase 2 contains a complex number will affect the analysis of the network.

项目成果

Menatetrenone, a vitamin K2 analogue, inhibits hepatocellular carcinoma cell growth by suppressing cyclin D1 expression through inhibition of nuclear factor kappaB activation.

Menatetrenone 是一种维生素 K2 类似物，通过抑制核因子 kappaB 激活来抑制细胞周期蛋白 D1 表达，从而抑制肝细胞癌细胞生长。

• 发表时间: 2007
• 期刊: Clinical Cancer Research 13
• 作者: Higashi Y;Nagasawa Okuda H;Swanson RA;Fujimoto S;Iwata Ozaki
• 通讯作者: Iwata Ozaki

Musashil modulates mammary progenitor cell expansion through proliferin-mediated activation of the Wnt and Notch pathways

• DOI: 10.1128/mcb.00040-08
• 发表时间: 2008-06-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Wang, Xiao-Yang;Yin, Yuzhi;Glazer, Robert I.
• 通讯作者: Glazer, Robert I.

ErbB2 induces Notch1 activity and function in breast cancer cells.

• DOI: 10.1111/j.1752-8062.2008.00041.x
• 发表时间: 2008-09
• 期刊: Clinical and translational science
• 作者: Lindsay J;Jiao X;Sakamaki T;Casimiro MC;Shirley LA;Tran TH;Ju X;Liu M;Li Z;Wang C;Katiyar S;Rao M;Allen KG;Glazer RI;Ge C;Stanley P;Lisanti MP;Rui H;Pestell RG
• 通讯作者: Pestell RG

Musashi1 modulates mammary progenitor cell expansion through proliferin-mediated activation of the Wnt and Notch pathways

Musashi1 通过增殖素介导的 Wnt 和 Notch 通路激活来调节乳腺祖细胞扩张

• 发表时间: 2008
• 期刊: Molecular and Cellular Biology Vol.28
• 作者: Wang XY;Yin Y;Yuan H;Sakamaki T;Okano H;Glazer RI.
• 通讯作者: Glazer RI.

ErbB2 Induces Notchl Activity and Function in Breast Cancer Cells.

ErbB2 诱导乳腺癌细胞中的 Notch1 活性和功能。

• 发表时间: 2008
• 期刊: Clinical and Translational Science 1
• 作者: Youichirou Higashi;Kazuki Nagasawa;Hiroto Okuda;Raymond A. Swanson;Sadaki Fujimoto;Jaime Lindsay
• 通讯作者: Jaime Lindsay