Mechanism of transformation of vascular smooth muscle cell-MicroRNA as regulating factor

非编码RNA中的微小RNA(MiRNA)在基因转录后与靶mRNA结合，抑制翻译。已有研究表明，这种基因沉默机制广泛存在于从植物到哺乳动物的各种物种中。尤其是miRNA被认为在细胞分化过程中起着重要的作用。血管平滑肌细胞(VSMC)的两种表型(收缩和增殖)的基因表达调控尚不清楚。我们发现，血小板衍生生长因子(PDGF)和丁酸钠(NAB)分别刺激VSMC的去分化和分化。上述两种表型调控所需的基因表达调控还不是很清楚。我们利用VSMC的两种表型的基因芯片分别对这些药物进行诱导，发现上调的miRNA。两种miRNA根据去分化上调刺激细胞迁移。

The micro RNA (miRNA) of non-coding RNA bind to a target mRNA after transcription of the gene and restrain the translation. It has been clarified that this gene silencing mechanism is processed among various species from plant to mammalian widely. Especially, it is thought that miRNA plays an important role on the way of the cell differentiation. The regulation of gene expression for two phenotypes (contraction and proliferation) of vascular smooth muscle cell (VSMC) is not well known. We established that the platelet-derived growth factor (PDGF) and sodium butyrate (NaB) stimulated de-differentiation and differentiation of VSMC, respectively. The regulation of gene expression required for the above two phenotypic modulations is not well known. We found the up-regulated miRNA by using microarray from each two phenotype of VSMC to induce with these medicines. The two up-regulated miRNA according to the de-differentiation stimulated the cell migration.