Mechanism of transformation of vascular smooth muscle cell-MicroRNA as regulating factor

血管平滑肌细胞转化机制——MicroRNA作为调控因子

• 批准号: 19590240
• 负责人: NAKAMURA Akio
• 金额: $ 2.83万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590240/
• 关键词: 血管平滑筋; 細胞分化; 脱分化; マイクロRNA; 細胞遊走; マクロRNA; 形質転換; 分化・脱分化; プロテオミクス; リン酸化

The micro RNA (miRNA) of non-coding RNA bind to a target mRNA after transcription of the gene and restrain the translation. It has been clarified that this gene silencing mechanism is processed among various species from plant to mammalian widely. Especially, it is thought that miRNA plays an important role on the way of the cell differentiation. The regulation of gene expression for two phenotypes (contraction and proliferation) of vascular smooth muscle cell (VSMC) is not well known. We established that the platelet-derived growth factor (PDGF) and sodium butyrate (NaB) stimulated de-differentiation and differentiation of VSMC, respectively. The regulation of gene expression required for the above two phenotypic modulations is not well known. We found the up-regulated miRNA by using microarray from each two phenotype of VSMC to induce with these medicines. The two up-regulated miRNA according to the de-differentiation stimulated the cell migration.

非编码RNA中的微小RNA（miRNA）在基因转录后与靶mRNA结合并抑制翻译。已经阐明，这种基因沉默机制广泛存在于从植物到哺乳动物的各种物种中。特别是miRNA被认为在细胞分化过程中起着重要作用。血管平滑肌细胞（VSMC）收缩和增殖两种表型的基因表达调控尚不清楚。我们建立了血小板衍生生长因子（PDGF）和丁酸钠（NaB）刺激VSMC的去分化和分化，分别。上述两种表型调节所需的基因表达调控尚不清楚。利用微阵列技术从两种表型的VSMC中检测到药物诱导表达上调的miRNA。这两种表达上调的miRNA均促进了细胞的迁移。

项目成果

Calcium inhibition of Physarum myosin as examined by the recombinant heavy mero-myosin.

• DOI: 10.1007/978-4-431-38453-3_22
• 发表时间: 2007
• 期刊: Advances in experimental medicine and biology
• 作者: H. Kawamichi;Ying Zhang;Mizuki Hino;A. Nakamura;Hideyuki Tanaka;L. Farkas;L. Nyitray;K. Kohama

Determination of phosphorylation site of smooth muscle myosin light chain kinase by various Ser/Thr kinases

各种 Ser/Thr 激酶对平滑肌肌球蛋白轻链激酶磷酸化位点的测定

• 发表时间: 2007
• 作者: Nakamura;A.;Kishi;H.;Matsumoto;A.;Ishikawa;R.;Kobayashi;S.;Kohama;K.
• 通讯作者: K.

Int.Rev.Cell Mol.Biol. 276(Cell and molecular biology of the fastest myosins)

Int.Rev.Cell Mol.Biol。

• 发表时间: 2009
• 作者: Higashi-Fujime;S.;Nakamura;A.
• 通讯作者: A.

Insulin signaling of diabetic mother rats and their offspring

糖尿病母鼠及其后代的胰岛素信号传导

• 发表时间: 2010
• 作者: Nasu;R.;Kohama;T.;Nakamura;A.;Kohama;K.
• 通讯作者: K.

Actin-bundles as a transporter of nano-sized cargoes

肌动蛋白束作为纳米级货物的运输者

• 发表时间: 2008
• 作者: Yoshiyama S.;Ishikawa R.;Nakamura A.;Higashi-Fujime S.;Takatsuki H.;Rice K.;Blough E. R.;Kohama K.
• 通讯作者: Kohama K.