The mechanism of regulation of metastasis and alteration in cell matrix by snail, transcription factor induce epithelilal -mesenchmal transduction

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We show that the expression of snail in epithelial MDCK and A431 cells enhance both cell detachment and attachment. Snail enhanced cell attachment to ECMs such as fibronectin. Snail also enhanced MDCK cell migration towards osteopontin that are ligands for integrin aVb3. We confirmed the reduction of laminin a3, b3 and g2 (Laminin-5) and of receptorsfor Laminin-5 such as integrins a3, a6 or b4 in MDCK/snail or in A431/snail cells. These results suggest that snail enhances cell detachment by multiple mechanism, and leads cell migration and reattachment at a second site, at least in part, by changing the expression of integrins in the cells.