The mechanism of regulation of metastasis and alteration in cell matrix by snail, transcription factor induce epithelilal -mesenchmal transduction

蜗牛、转录因子诱导上皮-间质转导调节转移和细胞基质改变的机制

• 批准号: 19590313
• 负责人: HARAGUCHI Misako
• 金额: $ 2.91万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590313/
• 关键词: 分子腫瘍学; 細胞骨格; 転移; snail; E-cadherin; EMT; 接着; スネイル; インテグリン; 細胞外基質; ラミニン; オステオポンチン

We show that the expression of snail in epithelial MDCK and A431 cells enhance both cell detachment and attachment. Snail enhanced cell attachment to ECMs such as fibronectin. Snail also enhanced MDCK cell migration towards osteopontin that are ligands for integrin aVb3. We confirmed the reduction of laminin a3, b3 and g2 (Laminin-5) and of receptorsfor Laminin-5 such as integrins a3, a6 or b4 in MDCK/snail or in A431/snail cells. These results suggest that snail enhances cell detachment by multiple mechanism, and leads cell migration and reattachment at a second site, at least in part, by changing the expression of integrins in the cells.

我们发现，蜗牛在上皮性MDCK和A431细胞中的表达增强了细胞的脱离和附着。蜗牛增强细胞对纤维连接蛋白等细胞外基质的附着。Snail还促进MDCK细胞向骨桥蛋白迁移，骨桥蛋白是整合素aVb3的配体。我们证实在MDCK/Snail或A431/Snail细胞中层粘连蛋白A3、b3和G2(层粘连蛋白-5)和层粘连蛋白-5受体如整合素A3、a6或b4减少。这些结果表明，蜗牛通过多种机制促进细胞脱离，并至少部分通过改变细胞内整合素的表达，导致细胞在第二个部位迁移和再附着。

项目成果

転写因子スネイルによるMDCK細胞の代謝調節

转录因子SNAIL对MDCK细胞的代谢调节

• 发表时间: 2009
• 作者: Haraguchi;M.;Okubo;T.;Miyashita;Y.;et al.;原口みさ子
• 通讯作者: 原口みさ子

転写因子スネイルはインテグリンや細胞外マトリックスタンパクの発現を調節することにより細胞と基質間の接着を制御する

转录因子 SNAIL 通过调节整合素和细胞外基质蛋白的表达来控制细胞基质粘附。

• 发表时间: 2007
• 作者: Haraguchi;M.;Okubo;T.;Miyashita;Y.;et al.;原口みさ子;原口みさ子;原口みさ子;原口みさ子
• 通讯作者: 原口みさ子

The role of the transcriptional in cell detachment, reattachment and Migration

转录在细胞分离、重新附着和迁移中的作用

• 发表时间: 2009
• 期刊: Adhesion &Migration 3
• 作者: Haraguchi;M.
• 通讯作者: M.

Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins

• DOI: 10.1074/jbc.m801125200
• 发表时间: 2008-08-29
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Haraguchi, Misako;Okubo, Tadashi;Ozawa, Masayuki
• 通讯作者: Ozawa, Masayuki

The role of transcriptional regulator snail in cell detachment, reattachment and migration.

转录调节因子 snail 在细胞分离、重新附着和迁移中的作用。

• 发表时间: 2009
• 期刊: Cell adhesion & migratio 3(3)
• 作者: 山根拓也;渡辺一史;杉本直之;蜂須麗;竹内圭介;前田利長;大久保岩男;山根 拓也;*Haraguchi M
• 通讯作者: *Haraguchi M