Investigation of the regulatory mechanisms of cellular energy metabolism by snail

蜗牛细胞能量代谢调节机制的研究

• 批准号: 22590287
• 负责人: HARAGUCHI Misako
• 金额: $ 2.91万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590287/
• 关键词: Snail; ピルビン酸デヒドロゲナーゼキナーゼ(PDK1); VLCAD; ATPクエン酸リアーゼ; ATP; グルコース; snail; ピルビン酸デヒドロゲナーゼ; MDCK; スネイル; ピルビン酸デヒドロゲナーゼキナーセ; ワールブルグ効果; 酸化的リン酸化; 解糖系

Snail expression decreases oxidative phosphorylation and increases glucose dependency in MDCK cells. MDCK/Snail cells exhibited reduced activity of pyruvate dehydrogenase (PDH), which controls pyruvate entry into the TCA cycle, and increased promoter activity and expression of pyruvate dehydrogenase kinase 1 (PDK1), which inhibits PDH. In addition, the activities of TCA cycle enzymes were decreased in MDCK/snail cells. Consequently, MDCK/snail cells exhibited lower O2 consumption and ATP levels than MDCK/neo cells. MDCK/snail cells also showed reduced expression of enzymes essential for glutaminolysis and fatty acid synthesis.

Snail 表达会降低 MDCK 细胞中的氧化磷酸化并增加葡萄糖依赖性。 MDCK/Snail 细胞表现出控制丙酮酸进入 TCA 循环的丙酮酸脱氢酶 (PDH) 活性降低，以及抑制 PDH 的丙酮酸脱氢酶激酶 1 (PDK1) 的启动子活性和表达增加。此外，MDCK/snail细胞中TCA循环酶的活性降低。因此，MDCK/snail 细胞表现出比 MDCK/neo 细胞更低的 O2 消耗和 ATP 水平。 MDCK/蜗牛细胞还表现出谷氨酰胺分解和脂肪酸合成所必需的酶的表达减少。

项目成果

Snail reduces oxidative phosphorylation in MDCK cells

Snail 降低 MDCK 细胞的氧化磷酸化

• 发表时间: 2012
• 作者: Misako Haraguchi;Masayuki Ozawa
• 通讯作者: Masayuki Ozawa

Snail による細胞内代謝の新規調節機構

Snail细胞内代谢调控新机制

• 发表时间: 2012
• 作者: 原口みさ子;小澤政之
• 通讯作者: 小澤政之

The transcription factor Snail expressed in cutaneous squamous cell carcinoma induces epithelial-mesenchymal transition and down-regulates COX-2

• DOI: 10.1016/j.bbrc.2012.12.035
• 发表时间: 2013-01-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Shimokawa, Mitsuyoshi;Haraguchi, Misako;Ozawa, Masayuki
• 通讯作者: Ozawa, Masayuki

Snailによる細胞内代謝の新規調節機構

Snail细胞内代谢调控新机制

• 发表时间: 2012
• 作者: Misako Haraguchi;Masayuki Ozawa;Salah M.El Sayed;吉岡和晃;定 清直;Misako Haraguchi;Hiramoto K;金善美;吉岡 和晃;定清直;原口みさ子
• 通讯作者: 原口みさ子

The transcription factor Snail enhanced the degradation of E-cadherin and desmoglein 2 in oral squamous cell carcinoma cells

• DOI: 10.1016/j.bbrc.2012.12.060
• 发表时间: 2013-01-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kume, Kenichi;Haraguchi, Misako;Ozawa, Masayuki
• 通讯作者: Ozawa, Masayuki