Investigation of the molecular pathology of the differentiation mechanism by the Notch signaling pathway in colon carcinogenesis process.

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The aim of this study is to investigate the relation of Notch signaling pathway on colon carcinogenesis by using the rats colon carcinogenic model. We found that the expression of Notch1 in colon tumor was significantly increased compared to the normal mucosa. In contrast, the expression of Klf4 in colon tumor was significantly decreased compared the normal mucosa. These results suggested that increased expression of Notch1 and decreased expression of Klf4 could be associated with the rat colon carcinogenesis. We also examined the identification of mucin-depleted foci (MDF) by using alcian blue pH2.5 stain in human colon mucosa of 57 subjects. We found that MDF existed in human colon mucosa. Histologically, MDF and OLL (Overlapped lesion with ACF) recognized the loss of goblet cells, nucleus swelling, nuclear stratification, and infiltrate of lymphocyte to stroma. The some of OLL represented the crypt with serrated change. These results suggested that MDF existed even in humans and it may be preneoplastic lesions and could be useful biomarker of human colon carcinogenesis.