Microarray analysis of host gene-expression during intra-cellular nests formation of Leishmania amastigotes.

利什曼原虫无鞭毛体细胞内巢形成过程中宿主基因表达的微阵列分析。

• 批准号: 19590431
• 负责人: MIMORI Tatsuyuki
• 金额: $ 2.91万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590431/
• 关键词: マイクロアレイ; 感染症; シグナル伝達; 遺伝子; 宿主細胞

We observed invading amastigote of Leishmania amazonensisto macrophage host cells with microscope, and performed the temporary changes of the host cell with cDNA microarray. Total RNA was separated from the non-infected host cell which a mouse macrophage cell strain (J774A.1), infected cells in early stages of protozoan infection, in the infectious formation period, and parasites. Those samples were analyzed about 41,252 probes using the Oligo-microarray system (Agilent Technologies) (4 X 44K) 1 color method.

用显微镜观察亚马逊利什曼原虫无鞭毛体侵入巨噬细胞宿主细胞的过程，用基因芯片检测宿主细胞的暂时性变化。从未感染的宿主细胞（小鼠巨噬细胞株（J774A.1））、在原生动物感染的早期阶段、在感染形成期的感染细胞和寄生虫中分离总RNA。使用Oligo-微阵列系统（Agilent Technologies）（4 X 44 K）1色方法分析这些样品约41，252个探针。