The investigation on expression and regulation of pathogenic invasive gene in dermatophytes using new transformation system.

利用新型转化系统研究皮肤癣菌致病侵入基因的表达和调控。

• 批准号: 19590457
• 负责人: MAKIMURA Koichi
• 金额: $ 2.91万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590457/
• 关键词: 病原性; 白癬菌; 動物モデル; 形質転換; 病原因子; 遺伝子破壊; 遺伝子診断; 白癬; プロテアーゼ; 選択マーカ; アグロバクテリウム法

Recently the number of patients affected by dermatophytosis are estimated more than 20% in Japan. For investigation on invasive (pathogenic) factor of dermatopytes, development of methods for destruction of target gene of the fungus is essential. In this study we constructed the original methods which are useful for investigation of invasive factor gene in major dermatophytes ; Trichophyton mentagrophytes using animal infectious models. At first we reported the new gene transformation system (Agrobacterium method) using Agrobacterium tumefaciens. Next we construct G418 as the marker of the multiple genes transformation. Finally we developed the Tmku80-destroyed Trichophyton, #49 strain. This strain has destroyed gene Tmku80 which is homolog of ku80 gene of T. mentagrophytes TIMM2789. By making #49 strain, specific gene destruction became possible, and also we could select target gene freely. At the same time we could compare the pathogenesis between modified gene strain and wild type strain of Trichophyton using experimental animal infectious models. Continuously we developed and reported a gene diagnosis system which is useful for human dermatophytosis. The result of this study would contribute to the analysis of clinical condition of dermatophytosis and progress of protection, diagnosis and therapy of the infection.

最近，在日本，受皮肤癣影响的患者人数估计超过20%。为了深入研究皮肤真菌的侵袭（致病）因子，开发破坏真菌靶基因的方法至关重要。在本研究中，我们构建了对主要皮肤真菌侵袭因子基因的初步研究方法；用动物感染模型研究毛癣菌。本文首次报道了以农杆菌为载体的新基因转化体系（农杆菌法）。接下来我们构建G418作为多基因转化的标记物。最后，我们开发了tmku80摧毁的毛癣菌，#49菌株。该菌株破坏了与T. mentagrophytes TIMM2789同源的ku80基因Tmku80。通过制造49号菌株，使基因的特异性破坏成为可能，并可自由选择靶基因。同时利用实验动物感染模型对毛癣菌基因修饰菌株与野生型菌株的发病机制进行了比较。我们不断开发并报道了一种对人类皮肤真菌病有用的基因诊断系统。本研究的结果将有助于分析皮肤真菌病的临床情况，以及对感染的保护、诊断和治疗的进展。

项目成果

Efficacy of Itraconazole in the Treatment of Patients with Chronic Cough Whose Sputa Yield Basidiomycetous FungiFungus-Associated Chronic Cough (FACC)

• DOI: 10.1080/02770900902846331
• 发表时间: 2009-01-01
• 期刊: JOURNAL OF ASTHMA
• 影响因子: 1.9
• 作者: Ogawa, Haruhiko;Fujimura, Masaki;Makimura, Koichi
• 通讯作者: Makimura, Koichi

Rapid identification of Prototheca zopfii by nested polymerase chain reaction based on the nuclear small subunit ribosomal DNA.

基于核小亚基核糖体 DNA 的巢式聚合酶链反应快速鉴定 Prototheca zopfii。

• 发表时间: 2009
• 期刊: Journal of Dermatological Science 54(1)
• 作者: Onozaki M;Makimura K;Hasagawa A
• 通讯作者: Hasagawa A

Efficacy of itraconazole in the treatment of patients with chronic cough whose sputa yield basidiomycetous fungi

伊曲康唑治疗痰中带担子菌的慢性咳嗽患者的疗效观察

• 发表时间: 2009
• 期刊: fungus-associated chronic cough (FACC) Journal of Asthma 46(4)
• 作者: Ogawa H;Fujimura M;Takeuchi Y;Makimura K
• 通讯作者: Makimura K

The importance of basidiomycetous fungi cultured from sputum of chronic idiopathic cough

慢性特发性咳嗽痰培养担子菌的重要性

• 发表时间: 2009
• 期刊: Respiratory Medicine 103(10)
• 作者: Ogawa H;Fujimura M;Takeuchi Y;Makimura K
• 通讯作者: Makimura K

打ち抜き感染(Breakthrough infection)として発症した侵襲性ムコール症の1剖検例.

尸检发现的侵袭性毛霉菌病是一种突破性感染。

• 发表时间: 2009
• 期刊: The Japanese Journal of Antibiotics 62(1)
• 作者: Mirhendi H;Moazeni M;Nikaeen M;Makimura K.;Onozaki M;Koga H;Yamada T;Yamamura M;AlShahni MM;Uchida T;Ogawa H;Ogawa H;AlShahni MM;Ebihara M;Sugita T;Ogawa H;Yamamura M;Yamada T;Kimura M;Miyajima Y;Satoh K;Mirhendi H;塩田量子;佐藤公美子;石原晋
• 通讯作者: 石原晋