Construction of efficient expression system of anti-tumor marker protein-monoclonal antibodies in transgenic plant.

转基因植物抗肿瘤标志蛋白-单克隆抗体高效表达体系的构建

• 批准号: 19590573
• 负责人: SATOH Hiroyuki
• 金额: $ 2.83万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590573/
• 关键词: 肝細胞癌; αフェトプロテイン; PIVKA-II; 遺伝子組換え植物; ペルオキシダーゼ; モノクローナル抗体; 腫瘍マーカー検査; AFP; plantibody; 腫瘍マーカー; ワサビ

To reduce the production cost of monoclonal antibody for diagnosis, we tried to express gamma-globulin cDNAs of AFP and PIVKA-II in transgenic plant for the diagnosis of hepatocellular carcinoma. After we cloned the cDNAs, construction of cDNAs into scFv was made for its expression in transgenic-plant. As the other approach, we found some novel peroxidases possess higher activity from Japanese wsabi radish than that of horse radish peroxidase.

为了降低诊断单克隆抗体的生产成本，我们试图表达转基因植物中AFP和PIVKA-II的γ-球蛋白CDNA，以诊断肝细胞癌的诊断。克隆cDNA后，将cDNA的构造在SCFV中构造是因为其在转基因植物中的表达。作为另一种方法，我们发现一些新型的过氧化物酶具有日本Wsabi萝卜的活性高于马萝卜过氧化物酶。

项目成果

A Proteomic Profiling Approach to Reveal a Novel Role of BnD22 (Brassica napus drought 22 kD)/Water-Soluble Chlorophyll binding Protein in young leaves during Nitrogen Remobilization induced by Stressful conditions

蛋白质组学分析方法揭示 BnD22（甘蓝型油菜干旱 22 kD）/水溶性叶绿素结合蛋白在胁迫条件诱导的氮再动员过程中幼叶中的新作用

• 发表时间: 2008
• 期刊: Plant Physiology 147
• 作者: Desclos;et al.
• 通讯作者: et al.

ニホンワサビペルオキシダーゼのcDNAクローニング

日本辣根过氧化物酶的cDNA克隆

• 发表时间: 2008
• 作者: 小嶋拓也;他
• 通讯作者: 他