The effect of exposure to chemical substance with bacterial toxin on the development of Th1/Th2 balance in infant mice.

接触含有细菌毒素的化学物质对幼年小鼠 Th1/Th2 平衡发育的影响。

• 批准号: 19590612
• 负责人: YAMAMOTO Shoji
• 金额: $ 2.83万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590612/
• 关键词: 環境生理; 小児健康影響; アレルギー; 感染症; 細菌; 免疫学; 有害化学物質

The aims of the present study were to clarify whether the respiratory tract infection and stimulation can influence development of Th1 immune response and allergic reaction or not, and to investigate the effects of co-stimulation of the respiratory tract infectious agent with toluene which is one of the causative factors for chemical sensitivity and sick house syndrome can affect Th1/Th2 balance in infant mice using an allergic mouse model. Our findings indicate that (1) respiratory tract infection and stimulation with cell wall component of gram positive bacteria, peptidoglycan (PGN) does not affect development of Th1 immune response and allergic reaction in infant mice with ovalbumin (OVA) immunization ; (2) subcutaneous inoculation with BCG vaccine enhanced Th1 response via increased plasma IgG2a antibody and IFN-γexpression in lung and spleen of OVA immunized mice (this finding clearly shows that although BCG inoculation did not induce respiratory tract stimulation, it can be used for induction of Th1 response) ; (3) low level toluene exposure only from fetal to neonatal stage may enhance the systemic Th2 function in infant mice, however, the co-exposure of low level toluene with PGN shifted Th1/Th2 immunity to suppressive direction ; (4) in addition, the co-exposure of low level toluene with PGN in infant stage altered both Th1 and Th2 dependent blood Ig antibody level in 3 week-old mice.

本研究的目的是阐明呼吸道感染和刺激是否会影响Th 1免疫应答和过敏反应的发展，并探讨呼吸道感染因子与化学敏感性和病屋综合征的致病因素之一甲苯的共同刺激对过敏小鼠模型中Th 1/Th 2平衡的影响。结果表明：（1）呼吸道感染和革兰氏阳性菌细胞壁成分肽聚糖（PGN）刺激不影响卵清蛋白（OVA）免疫幼鼠产生Th 1免疫应答和变态反应;（2）皮下接种卡介苗可通过增加OVA免疫小鼠血浆IgG 2a抗体和肺、脾IFN-γ的表达，增强Th 1应答（3）仅在胎鼠至新生鼠阶段，低浓度甲苯暴露可增强幼鼠全身性Th 2免疫功能，而低浓度甲苯与PGN联合暴露则使Th 1/Th 2免疫向抑制方向移动;（4）低浓度甲苯与PGN在婴儿期联合暴露，可使3周龄小鼠Th 1和Th 2依赖性IG抗体水平发生变化。

项目成果

低濃度トルエン曝露が発達期のマウス免疫系に及ぼす影響

低浓度甲苯暴露对小鼠免疫系统发育的影响

• 发表时间: 2008
• 作者: Kotani K;Sakane N;Tsuzaki K;Matsuoka Y;Sano Y;Hamada T;Yamada K;山元昭二・藤巻秀和
• 通讯作者: 山元昭二・藤巻秀和

Effect of long-term exposure to low-level toluene on airway inflammatory response in mice

• DOI: 10.1016/j.toxlet.2006.11.008
• 发表时间: 2007-01-30
• 期刊: TOXICOLOGY LETTERS
• 影响因子: 3.5
• 作者: Fujimaki, Hidekazu;Yamamoto, Shoji;Arashidani, Keiichi
• 通讯作者: Arashidani, Keiichi

揮発性有機化合物の胎仔期曝露による仔マウスのTh1, Th2型免疫応答のかく乱

由于胎儿暴露于挥发性有机化合物，幼鼠的 Th1 和 Th2 型免疫反应受到破坏

• 发表时间: 2010
• 期刊: 岡山実験動物研究会報誌 26
• 作者: Wang CY.;et al.;山元昭二・Tin-Tin-Win-Shwe・藤巻秀和
• 通讯作者: 山元昭二・Tin-Tin-Win-Shwe・藤巻秀和

Increased hippocampal mRNA expression of neuronal synaptic plasticity related genes in mice chronically exposed to toluene at a low-level human occupational-exposure.

在低水平人类职业暴露下长期接触甲苯的小鼠中，神经元突触可塑性相关基因的海马 mRNA 表达增加。

• 发表时间: 2007
• 期刊: Neurotoxicology 28・1
• 作者: Sohel Ahmed

Children's Immunology, what can we learn from animal studies (2): Modulation of systemic Th1/Th2 immune response in infant mice after prenatal exposure to low-level toluene and toll-like receptor (TLR) 2 ligand

• DOI: 10.2131/jts.34.sp341
• 发表时间: 2009-07-01
• 期刊: JOURNAL OF TOXICOLOGICAL SCIENCES
• 影响因子: 2
• 作者: Yamamoto, Shoji;Tin-Tin-Win-Shwe;Fujimaki, Hidekazu
• 通讯作者: Fujimaki, Hidekazu