Identification of Genomic Locus Related to Sensitivity Against Anticancer Drug and Predisposition of Pancreatic Cancer Using Comprehensive Analysis

综合分析鉴定与抗癌药物敏感性和胰腺癌易感性相关的基因组位点

• 批准号: 19590751
• 负责人: SASAHIRA Naoki
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590751/
• 关键词: 膵臓病学; 高密度SNPアレイ; 膵癌; 高密度オリゴヌクレオチドアレイ; 遺伝子増幅; LOH(ヘテロ接合性の消失); 胆道癌; CYP2C19; 欠失

We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array and found eight amplified regions and eight deleted regions in at least 2 cell lines. We then examined representative genes at the eight amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. Then we analyzed the relationship between SNP of cytochrome P450 and the susceptibility of pancreatic cancer. Genetic polymorphism of CYP2C19 was associated with the susceptibility to pancreatic cancer, but it was not significant statistically. In the case of bile duct cancer, it was proved to be significant.

我们使用高密度单核苷酸多态性 (SNP) 阵列分析了 25 个已建立的胰腺癌细胞系中的全基因组拷贝数改变和杂合性丢失 (LOH)，并在至少 2 个细胞系中发现了 8 个扩增区域和 8 个缺失区域。然后，我们检查了配对的胰腺癌和正常组织中八个扩增基因座的代表性基因。 14 个显微解剖组织样本中的 1 个（7.1%）至 5 个（35.7%）检测到扩增。然后我们分析了细胞色素P450的SNP与胰腺癌易感性的关系。 CYP2C19基因多态性与胰腺癌易感性相关，但无统计学意义。在胆管癌的例子中，它被证明是显着的。

项目成果

Integrated analysis of copy number alterations and loss of heterozygosity in human pancreatic cancer using a high-resolution, single nucleotide polymorphism array

• DOI: 10.1159/000155813
• 发表时间: 2008-01-01
• 期刊: ONCOLOGY
• 影响因子: 3.5
• 作者: Lin, Lian-Jie;Asaoka, Yoshinari;Omata, Masao
• 通讯作者: Omata, Masao

膵特異的遺伝子改変マウス膵発癌モデルを用いた膵癌の新規分子標的治療の検討

使用胰腺特异性基因修饰的小鼠胰腺癌发生模型研究胰腺癌的新型分子靶向治疗

• 发表时间: 2009
• 作者: 伊地知秀明;多田稔;ほか
• 通讯作者: ほか

膵検体による遺伝子診断

使用胰腺样本进行基因诊断

• 发表时间: 2007
• 期刊: 医学のあゆみ 222巻
• 作者: Lin L. J.;et. al.;多田稔
• 通讯作者: 多田稔

Risk of pancreatic cancer in calcified chronic pancreatitis

钙化慢性胰腺炎患胰腺癌的风险

• 期刊: J Gastroenterol (in press)
• 作者: 笹平直樹;多田稔;ほか
• 通讯作者: ほか

A genetic polymorphism of CYP2C19 is associated with the susceptibility to bile duct cancer.

CYP2C19的基因多态性与胆管癌的易感性相关。

• 发表时间: 2009
• 作者: 磯村好洋;多田稔;ほか;磯村好洋
• 通讯作者: 磯村好洋