Identification of Genomic Locus Related to Sensitivity Against Anticancer Drug and Predisposition of Pancreatic Cancer Using Comprehensive Analysis

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We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array and found eight amplified regions and eight deleted regions in at least 2 cell lines. We then examined representative genes at the eight amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. Then we analyzed the relationship between SNP of cytochrome P450 and the susceptibility of pancreatic cancer. Genetic polymorphism of CYP2C19 was associated with the susceptibility to pancreatic cancer, but it was not significant statistically. In the case of bile duct cancer, it was proved to be significant.