The role of the novel calcium-responsive transcription factors in kerationocyte proliferation and differentiation

新型钙反应性转录因子在角质细胞增殖和分化中的作用

• 批准号: 19591296
• 负责人: KAWACHI Yasuhiro
• 金额: $ 2.83万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591296/
• 关键词: 表皮; 角化; 転写因子; YY1; ケラチノサイト; 4BP4; 表皮角化細胞; 細胞分化

Yin Yang-1(以下YY-1)は偏在する転写因子であり、様々な転写制御に関わっていると言われています。YY-1蛋白は多くの組織や細胞で同定されており、またその働きは組織や細胞で異なります。我々は以前YY-1蛋白が表皮基底層で強く発現され、上層に行くにつれて弱くなることを確認しました。この結果を元に、YY-1が表皮角化細胞の分化過程により重要な役割を担っているのではないかと考え、新たに3D培養法という手法と、角化細胞のセルラインであるHaCaT細胞を用い、研究を行いました。結果として、過発現したYY-1は3D培養において、表皮の著明な肥厚、分化の抑制、未分化状態の維持、及び分裂能の増加などを示しました。これらは、通常の皮膚組織において、YY-1が表皮基底層における角化細胞の未分化状態の維持と、上層での分化移行に重要な働きを示唆しています。

Yin Yang-1 (the following YY-1) is biased to write about the factor and write the information to make sure that you don't know what to say. The expression of YY-1 protein in multiplex tissue was the same as that in normal tissue and normal tissue. We checked the previous YY-1 protein epidermis base plate to make sure that there was no significant change in the epidermis base, and that the previous epidermis base layer of the epidermis protein had a strong effect on the epidermal substrate. The results showed that the process of cell differentiation of YY-1 epidermis keratosis was an important step in the process of cell differentiation. The results showed that the process of cell differentiation of YY-1 epidermis keratosis was important in the process of differentiation of epidermal keratosis cells. Results the results showed that the epidermis was hypertrophy, the differentiation was inhibited, the undifferentiated status was maintained, and the mitotic energy was increased. The results showed that the epidermis was hypertrophy, the differentiation was inhibited, the undifferentiated status was maintained, and the mitotic energy was added to the YY-1. The skin tissue, the normal skin tissue, the YY-1 epidermis, the epidermis, the keratinized cells, the undifferentiated cells, the differentiated cells, the differentiated cells, the cells, the cells,

项目成果

The induction of tumor‐specific CD4+ T cells via major histocompatibility complex class II is required to gain optimal anti‐tumor immunity against B16 melanoma cell line in tumor immunotherapy using dendritic cells

• DOI: 10.1111/j.1600-0625.2008.00802.x
• 发表时间: 2009-04
• 期刊: Experimental Dermatology
• 影响因子: 3.6
• 作者: Y. Fujisawa;T. Nabekura;Tomohei Nakao;Yasuhiro Nakamura;Takenori Takahashi;Y. Kawachi;F. Otsuka;M. Onodera

hyperplasia with underlying epidermal growth factor-producing cutaneous CD30-positive lymphoproliferative disorder

增生伴有潜在的产生表皮生长因子的皮肤 CD30 阳性淋巴组织增生性疾病

• 发表时间: 2008
• 期刊: J Eur Acad Dermatol Venereol 23
• 作者: Kawachi Y;Taguchi S;Fujisawa Y;Furuta J;Nakamura Y;Ishii Y;Takahashi T;Otsuka F.
• 通讯作者: Otsuka F.

F : The combination of ubiquitous transcription factors AP-1 and Sp1 directs keratinocyte-specific and differentiation-specific gene expression in vitro

F : 普遍存在的转录因子 AP-1 和 Sp1 的组合在体外指导角质形成细胞特异性和分化特异性基因表达

• 发表时间: 2007
• 期刊: Expimental Dermatology 16
• 作者: Nakamura Y;Kawachi Y;Xu X;Sakurai H;Ishii Y;Takahashi T;Otsuka F
• 通讯作者: Otsuka F

Attenuation of UVB-Induced Sunburn Reaction and Oxidative DNA Damage with No Alterations in UVB-induced Skin Carcinogenesis.

减弱 UVB 引起的晒伤反应和氧化 DNA 损伤，但不改变 UVB 引起的皮肤癌发生。

• 发表时间: 2008
• 期刊: J Invest Dermatol 128
• 作者: Nakamura Y;Kawachi Y;Furuta J;Otsuka F.;Kawachi Y et al.
• 通讯作者: Kawachi Y et al.

Epidermal cell necrosis with direct epidermal infiltration of EB-ER-positive T lymphocytes in a patient with Epstein-Barr-virus-associated hemophagocytic syndrome

EB 病毒相关噬血细胞综合征患者表皮细胞坏死伴 EB-ER 阳性 T 淋巴细胞直接表皮浸润

• 发表时间: 2007
• 期刊: British Journal of Dermatology 157
• 作者: Kawachi Y;Itoh M;Fujisawa Y;Banno T;Takahashi T;Otsuka F
• 通讯作者: Otsuka F