The significance of prostaglandin in epithelial-mesenchymal transition in pathophysiology of gastrointestinal disease

前列腺素（PG）通过促进细胞增殖、迁移、侵袭和转移，在胃肠道创伤愈合和癌变过程中发挥重要作用。为探讨上皮细胞向间质细胞转化（EMT）和前列腺素（PG）在胃肠道疾病病理生理中的作用，本研究探讨了胃癌和胃溃疡组织中15-羟前列腺素脱氢酶（15-PGDH）的变化.我们确定了胃腺癌中15-PGDH表达模式与各种临床病理因素和患者预后之间的相关性。71例胃腺癌组织中35例15-PGDH蛋白表达降低。多因素分析显示15-PGDH表达降低是生存不良的独立预测因子.我们研究了15-PGDH在小鼠胃和乙酸诱导的胃溃疡中的表达，以及胃溃疡愈合过程中15-PGDH的调节机制。与正常组织相比，PGDH的mRNA表达降低了57%。与此一致，与正常组织相比，溃疡组织中15-PGDH的蛋白水平降低。表皮生长因子受体（EGFR）激酶抑制剂可逆转溃疡组织中15-PGDH mRNA表达的下调。

Prostaglandin (PG) plays an important role in gastrointestinal wound healing and carcinogenesis by enhancing cell proliferation, migration, invasion and metastasis. In the preliminary study for the research of epithelial to mesenchymal transition (EMT) and PG in pathophysiology of gastrointestinal disease, we investgated the alteration of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in gastric cancer and gastric ulcer.1. We determined the correlations between pattern of expression of 15-PGDH in gastric adenocarcinoma and various clinicopathological factors and patient outcome. In 35 of 71 cases of gastric adenocarcinoma, expression of 15-PGDH protein was reduced in tumor tissues. Multivariate analysis revealed reduction of 15-PGDH expression to be an independent predictor of poor survival.2. We investigated the expression of 15-PGDH in the stomach and acetic acid-induced gastric ulcer in mice and the mechanism by which 15-PGDH is regulated during gastric ulcer healing. Expression of mRNA for PGDH was reduced by 57% compared with normal tissue. Consistent with this, protein level of 15-PGDH in ulcer tissue was decreased compared with that in normal tissue. Epidermal growth factor receptor (EGFR) kinase inhibitor reversed the down-regulation of mRNA expression of 15-PGDH in ulcer tissue.