The significance of prostaglandin in epithelial-mesenchymal transition in pathophysiology of gastrointestinal disease

前列腺素在上皮间质转化中胃肠道疾病病理生理学中的意义

• 批准号: 19790493
• 负责人: TANIGAWA Tetsuya
• 金额: $ 2.15万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19790493/
• 关键词: 上皮―間充織形質転換; プロスタグランジン; 15-hydroxyprostaglandin dehydrogenase; 創傷治癒; 胃癌; 上皮-間充織形質転換; 上皮-間充織形転換; 15-hdroxrostalandin dehydroenase; E-cadherin; a-smooth muscle actin; restitution

Prostaglandin (PG) plays an important role in gastrointestinal wound healing and carcinogenesis by enhancing cell proliferation, migration, invasion and metastasis. In the preliminary study for the research of epithelial to mesenchymal transition (EMT) and PG in pathophysiology of gastrointestinal disease, we investgated the alteration of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in gastric cancer and gastric ulcer.1. We determined the correlations between pattern of expression of 15-PGDH in gastric adenocarcinoma and various clinicopathological factors and patient outcome. In 35 of 71 cases of gastric adenocarcinoma, expression of 15-PGDH protein was reduced in tumor tissues. Multivariate analysis revealed reduction of 15-PGDH expression to be an independent predictor of poor survival.2. We investigated the expression of 15-PGDH in the stomach and acetic acid-induced gastric ulcer in mice and the mechanism by which 15-PGDH is regulated during gastric ulcer healing. Expression of mRNA for PGDH was reduced by 57% compared with normal tissue. Consistent with this, protein level of 15-PGDH in ulcer tissue was decreased compared with that in normal tissue. Epidermal growth factor receptor (EGFR) kinase inhibitor reversed the down-regulation of mRNA expression of 15-PGDH in ulcer tissue.

前列腺素（PG）通过促进细胞增殖、迁移、侵袭和转移，在胃肠道创伤愈合和癌变过程中发挥重要作用。为探讨上皮细胞向间质细胞转化（EMT）和前列腺素（PG）在胃肠道疾病病理生理中的作用，本研究探讨了胃癌和胃溃疡组织中15-羟前列腺素脱氢酶（15-PGDH）的变化.我们确定了胃腺癌中15-PGDH表达模式与各种临床病理因素和患者预后之间的相关性。71例胃腺癌中有35例肿瘤组织中15-PGDH蛋白表达减少。多因素分析显示15-PGDH表达降低是生存不良的独立预测因子.我们研究了15-PGDH在小鼠胃和乙酸诱导的胃溃疡中的表达，以及胃溃疡愈合过程中15-PGDH的调节机制。与正常组织相比，PGDH的mRNA表达降低了57%。与此一致，与正常组织相比，溃疡组织中15-PGDH的蛋白水平降低。表皮生长因子受体（EGFR）激酶抑制剂可逆转溃疡组织中15-PGDH mRNA表达的下调。

项目成果

Reduction of 15-hydroxyprostaglandin dehydrogenase expression is an independent predictor of poor survival associated with enhanced cell proliferation in gastric adenocarcinoma

• DOI: 10.1111/j.1349-7006.2009.01390.x
• 发表时间: 2010-02-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Tatsuwaki, Hiroshi;Tanigawa, Tetsuya;Arakawa, Tetsuo
• 通讯作者: Arakawa, Tetsuo

胃潰瘍治癒過程におけるプロスタグランジン代謝酵素 : 15-ydroxyprostaglandin dehydrogenaseの発現動態について

胃溃疡愈合过程中的前列腺素代谢酶：15-羟基前列腺素脱氢酶的表达动态

• 发表时间: 2008
• 作者: 谷川徹也;ほか
• 通讯作者: ほか

胃癌の細胞増殖に対する15-Hydroxyprostaglandin dehydroge naseの意義

15-羟基前列腺素脱氢酶对胃癌细胞增殖的意义

• 发表时间: 2009
• 作者: 達脇大;他
• 通讯作者: 他

胃癌組織における15-Hydroxyprostaglandin dehydrogenaseの発現動態の検討

胃癌组织中15-羟基前列腺素脱氢酶表达动态的检测

• 发表时间: 2008
• 作者: 達脇大;谷川徹也;渡辺俊雄;町田浩久;岡崎博俊;斯波將次;富永和作;藤原靖弘;押谷伸英;六車一哉;澤田鉄二;平川弘聖;樋口和秀;荒川哲男
• 通讯作者: 荒川哲男

消化器疾患における分子腫瘍マーカーの新展開胃癌における15-Hydroxyprostaglandin dehydrogenaseの発現低下は独立予後不良予測因子である

胃肠道疾病分子肿瘤标志物新进展 胃癌中15-羟基前列腺素脱氢酶表达降低是预后不良的独立预测因子

• 发表时间: 2010
• 作者: 谷川徹也;達脇大;渡辺俊雄
• 通讯作者: 渡辺俊雄