A study of clinical utilization of glycoproteins as a early diagnostic marker of Alzheimer's disease.

我们以前发现，三个糖蛋白的糖基化改变阿尔茨海默病（AD）。在本研究中，我们鉴定了所有的糖蛋白，建立了每种糖蛋白的糖定量方法，并测量了它们的糖基化。与非痴呆对照组（NDC）相比，AD患者血清转铁蛋白和免疫系统相关蛋白中几种糖的含量发生了变化。Alzheimer病早期血清转铁蛋白中唾液酸含量也发生变化，提示转铁蛋白中唾液酸含量是一种有效的AD血清诊断标志物。免疫系统相关蛋白与NDC组和非AD痴呆组相比均存在糖基化改变，并与脑脊液淀粉样蛋白β（Aβ）相关。该糖蛋白有望成为监测脑内Aβ变化的有效血清标志物。

We previously discovered that the glycosylation of three glycoproteins were altered in Alzheimer's disease (AD). In this study, we identified all glycoproteins, established the methods to quantify the sugars of each glycoproteins and measured glycosylation of them. Identified serum transferrin and immune system associated protein had altered quantities of several kinds of sugar in AD patients compared with non-demented control (NDC) groups. Sialic acid in Alzheimer's serum transferrin also changed in early stage of AD, which suggests the sialic acid of transferrin is an available serum diagnostic marker of AD. Immune system associated protein had altered sugars compared with not only NDC groups but also non-AD dementia groups, and correlated with cerebrospinal fluid amyloid β(Aβ). This glycoprotein is expected to be an efficient serum marker which can monitor the changes of Aβ in the brain.