Establishment of the mesureing system for early diagnosis of Alzheimer's disease using blood biological markers.

本研究的目的是提纯阿尔茨海默病(AD)患者血清中的两种糖蛋白，分析其变化区域，并建立相应的检测系统。然而，我们在纯化它们的步骤中遇到了一些问题，例如，另一个糖链被目标糖蛋白污染。虽然我们无法进行分析，但我们最终确定了最合适的纯化蛋白质的方法，目前正在作为第一个计划推进。我们发现阿尔茨海默病患者血清中唾液酸结合转铁蛋白的唾液酸转移酶(STase)的含量和活性都较低。这可能是AD患者唾液酸改变的原因之一。

The purpose of this study was to purify two glycoproteins, which is altered in Alzheimer's disease (AD) serum, analyze the region changed in AD, and then establish the measuring system. However, we had the problems during the steps of purify them, for example another sugar chains contaminated in the target glycoproteins. Although we cannot progress analysis, we finally decided the most suitable way to purify the proteins and are now advancing as the first planning. And we found the both content and the activity of serum sialyltransferase (STase) which attach the sialic acid to the transferrin are lower in Alzheimer's serum. This indicates one of the causes about altered sialic acid in AD.