Analysis of peripheral blood hematopoietic stemness using human hTERT-stromal cells

使用人 hTERT 基质细胞分析外周血造血干细胞

• 批准号: 19790672
• 负责人: KAWANO Yutaka
• 金额: $ 2.36万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19790672/
• 关键词: 末梢血; 造血幹細胞; CD133; 骨髄間質細胞; hTERT

ヒトテロメレース遺伝子を導入したストローマ(以下HTS)との共培養により末梢血CD133陽性細胞は臍帯血CD34陽性細胞と同等に血液細胞の増幅が可能であった.末梢血CD133陽性細胞をHTS接触下あるいは非接触下で共培養を行い血液細胞の増幅に差を認めなかった.液性因子であるSCF、angiopoietin、およびWnt抑制因子を添加しながらHTSと共培養をおこなった.その結果、上記因子の添加有無で血液細胞の増幅や細胞周期へのentryは同等であった.

The co-culture of CD133 positive cells in peripheral blood and CD34 positive cells in umbilical blood may increase the amplitude of blood cells. CD133 positive cells in peripheral blood were cultured under contact with HTS and without contact. SCF, angiopoieton, Wnt inhibitors were added to the culture. The results, the addition of the above factors, the increase of blood cells, the entry of the cell cycle, and the like.

项目成果

Ex vivo expansion of G-CSF-mobilized peripheral blood CD133+ progenitor cells on coculture with human stromal cells.

• DOI: 10.1016/j.exphem.2005.10.007
• 发表时间: 2006-02
• 期刊: Experimental hematology
• 影响因子: 2.6
• 作者: Y. Kawano;M. Kobune;Hiroki Chiba;Kiminori Nakamura;R. Takimoto;K. Takada;Yoshinori Ito;J. Kato;H. Hamada;Y. Niitsu

Wnt3/RhoA/ROCK signaling pathway is involved in adhesion-mediated drug resistance of multiple myeloma in an autocrine mechanism

• DOI: 10.1158/1535-7163.mct-06-0684
• 发表时间: 2007-06-01
• 期刊: MOLECULAR CANCER THERAPEUTICS
• 影响因子: 5.7
• 作者: Kobune, Masayoshi;Chiba, Hiroki;Niitsu, Yoshiro
• 通讯作者: Niitsu, Yoshiro

Adenoviral vector-mediated transfer of the Indian hedgehog gene modulates lymphomyelopoiesis in vivo

• DOI: 10.1634/stemcells.2007-0741
• 发表时间: 2008-02-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Kobune, Masayoshi;Kato, Junii;Niitsu, Yoshiro
• 通讯作者: Niitsu, Yoshiro