nvestigation of the behavior of nuclear proteins in human cancer cells

目的：研究核仁蛋白和AgNORs蛋白在紫外线诱导口腔癌细胞凋亡中的行为。用免疫印迹分析和组织细胞化学方法检测紫外线诱导的SCC-25和HSG细胞核仁蛋白和AgNORs蛋白的动态变化。核仁蛋白和AgNORs蛋白的动态变化。口腔癌细胞的凋亡体中，核仁消失或弥漫分布于细胞核内。这一结果表明，核仁的改变与紫外线诱导口腔癌细胞DNA片段化或最终的凋亡活跃期有关。

To investigate the behavior of nucleolin and AgNORs proteins in ultra-violet ray (UV)-induced oral cancer apoptotic cells. Dynamic alteration of nucleolin and AgNORs proteins in UV-induced SCC-25 and HSG cells were examined using Western blot analysis and histocytochemistry methods. Dynamic alteration of nucleolin and AgNORs proteins are observed. Nucleolin disappeared or diffusely spread out into nucleus in apoptotic body of oral cancer cells. This results indicate that alteration of nucleolin is associated with the induction of DNA fragmentation or the final active phase of apoptosis induced by UV in oral cancer cells.