Development of a screening method for drugs in formalin-fixed tissue

福尔马林固定组织中药物筛选方法的开发

• 批准号: 19790451
• 负责人: TSUJIKAWA Kenji
• 金额: $ 1.56万
• 依托单位: National Research Institute of Police Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19790451/
• 关键词: 法医鑑定学; 薬毒物スクリーニング

The aim of this study is to develop a screening method for 38 central nervous system drugs such as hypnotics, antidepressants, antipsychotics. The drug standards were spiked to methanol homogenate of porcine liver, then clean-up was performed as following order : i) fat precipitation by cooling, ii) hexane-acetonitrile partition, iii) liquid-liquid extraction under basic condition. The sample prepared was determined by liquid chromatography tandem mass spectrometry. The recovery and repeatability (CV%) of the targeted drugs except for 7-aminobenzodiazepines (3 drugs) were more than 40% and within 15%, respectively. The method would be useful for drug screening in solid tissues.

本研究旨在建立催眠药、抗抑郁药、抗精神病药等38种中枢神经系统药物的筛选方法。将药物标准品加标至猪肝的甲醇匀浆中，然后按以下顺序进行净化：i）通过冷却进行脂肪沉淀，ii）己烷-乙腈分配，iii）在碱性条件下进行液-液萃取。制备的样品通过液相色谱串联质谱法测定。除7-氨基苯二氮卓类药物（3种）外，其他靶向药物的回收率和重复性（CV%）分别大于40%和15%。该方法可用于实体组织中的药物筛选。