Development of a screening method for drugs in formalin-fixed tissue

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The aim of this study is to develop a screening method for 38 central nervous system drugs such as hypnotics, antidepressants, antipsychotics. The drug standards were spiked to methanol homogenate of porcine liver, then clean-up was performed as following order : i) fat precipitation by cooling, ii) hexane-acetonitrile partition, iii) liquid-liquid extraction under basic condition. The sample prepared was determined by liquid chromatography tandem mass spectrometry. The recovery and repeatability (CV%) of the targeted drugs except for 7-aminobenzodiazepines (3 drugs) were more than 40% and within 15%, respectively. The method would be useful for drug screening in solid tissues.