The mechanism of multifunctional biological activity of Ganoderma lucidam with preventive effects of sex-hormone related disease

灵芝（灵芝，Mannentake或灵芝）在东亚已被用于治疗各种人类疾病数百年。本实验研究了灵芝三萜类化合物的体外抗雄激素作用和抑制成骨细胞分化作用。灵芝酸DM对512-还原酶的抑制作用最强。灵芝酸DM可抑制NF-κB配体受体激活因子（RANKL）和肿瘤坏死因子α（TNF-α）诱导的成骨细胞分化。灵芝酸DM具有抗雄激素和抗骨细胞分化的双重作用，这可以解释灵芝酸DM的多功能性。lucidum。这些三萜类化合物生物活性研究的积累，为进一步阐明灵芝未解的功能提供了线索。

The fungus Ganoderma lucidum (Reishi, Mannentake, or Lingzhi) has been used for centuries in East Asia to treat various human diseases. We investigated anti-androgen effect and the inhibitory effects of osteoclastic differentiation of triterpenoids isolated from G lucidum in vitro. Ganoderic acid DM showed the strongest 512-reductase inhibiting effect among the other ten triterpenoids. Also, ganoderic acid DM inhibited the osteoclastic differentiation, which responding to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor a (TNF-α). Ganoderic acid DM showed the dual functions such as anti-androgenic and anti-osteoclastic differentiation activities, which can be used to explain the multifunction of G. lucidum. The accumulation of the knowledge of biological activities of these kinds of triterpenoids leads us to give some hints in elucidating the unsolved function of G lucidum.