Transcruptional Regulation of Sox9 during endochondral ossification

软骨内骨化过程中 Sox9 的转录调控

• 批准号: 19689037
• 负责人: HATA Kenji
• 金额: $ 17.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19689037/
• 关键词: 口腔生化学; 軟骨細胞; 転写因子; Sox9

Most of bones were formed through unique ossification pathway called endochondral ossification. It is well known that transcriptional factor Sox9 is essential for chondrocyte differentiation. To understand the molecular basis for chondrogenesis, we established screening system which can allow us to identify transcriptional co-factor for Sox9. We have identified p54^<nrb> and Dmrt5 as a novel transcriptional partner for Sox9. Further studies revealed that p54^<nrb> modulate mRNA splicing of Sox9 target genes. Moreover, we found that Dmrt5 is involeved in Sox9 dependent sex differentiation as well as chondrocyte differentiation. Our findings indicate that the screening system is valid and the newly identified Sox9 transcriptional cofactor is important for chondrocyte differentiation.

大多数骨骼是通过被称为软骨内成骨的独特的成骨途径形成的。众所周知，转录因子Sox9对软骨细胞的分化是必不可少的。为了了解软骨形成的分子基础，我们建立了筛选体系，可以鉴定Sox9的转录辅助因子。我们已经确定p54^&lt；nrb&gt；和Dmrt5是Sox9的一个新的转录伙伴。进一步的研究表明，p54^&lt；nrb&gt；调节Sox9靶基因的mRNA剪接。此外，我们还发现Dmrt5参与了Sox9依赖的性别分化和软骨细胞的分化。我们的研究结果表明，该筛选系统是有效的，新发现的Sox9转录辅因子对软骨细胞的分化具有重要意义。

项目成果

Paraspeckle protein p54 links Sox9-mediated transcription with RNA processing during chondrogenesis in mice

Paraspeckle 蛋白 p54 将 Sox9 介导的转录与小鼠软骨形成过程中的 RNA 加工联系起来

• 发表时间: 2008
• 期刊: J Clin Invest 118
• 作者: Hata;K.,Nishimura;R.;Yoneda;T.;et.al.
• 通讯作者: et.al.

Sox9 family members negatively regulate maturation and calcification of chondrocytes through up-regulation of PTHrP.

Sox9 家族成员通过上调 PTHrP 负向调节软骨细胞的成熟和钙化。

• 发表时间: 2009
• 期刊: Mol Biol Cell 20
• 作者: Amano K;Hata K;Suigita A;Takigawa Y;Ono K;Wakabayashi M;Kogo M;Nishimura R;Yoneda T
• 通讯作者: Yoneda T

Sox9転写調節メカニズムの解明

阐明Sox9转录调控机制

• 发表时间: 2009
• 作者: Suematsu A;Nakashima T;波多賢二
• 通讯作者: 波多賢二

Transcription factor Dmrt2, a downstream molecule of Sox 5/6/9, promotes late chondrogenesis as a linker between Sox5/6/9 and Runx2

转录因子 Dmrt2 是 Sox 5/6/9 的下游分子，作为 Sox5/6/9 和 Runx2 之间的连接体促进晚期软骨形成

• 发表时间: 2009
• 作者: Ono K;Hata K;et al
• 通讯作者: et al

Zoledronic acid delays wound healing of the tooth extraction socket, inhibits oral epithelial cell migration, and promotes proliferation and adhesion to hydroxyapatite of oral bacteria, without causing osteonecrosis of the jaw, in mice

• DOI: 10.1007/s00774-009-0128-9
• 发表时间: 2010-03-01
• 期刊: JOURNAL OF BONE AND MINERAL METABOLISM
• 影响因子: 3.3
• 作者: Kobayashi, Yasuyoshi;Hiraga, Toru;Yoneda, Toshiyuki
• 通讯作者: Yoneda, Toshiyuki