Role of Sox9 transcriptional network during chondrocyte differentiation

Sox9转录网络在软骨细胞分化过程中的作用

• 批准号: 23792121
• 负责人: HATA Kenji
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792121/
• 关键词: 軟骨細胞; Sox9; 転写因子

Sox9 is a transcription factor critical for endochondral ossification; however, proof of its epigenetic regulation remains elusive. Here, we identified Arid5b (AT-rich interactive domain 5b) as a novel transcriptional co-regulator for Sox9. Arid5b physically interacted with Sox9 and cooperatively promoted chondrogenesis through direct binding to the Col2a1gene promoter. Arid5b^-/-mice showed growth retardation with delayed chondrogenesis. The methylation level of H3K9 in chondrogenic marker gene promoters was markedly increased in Arid5b^-/- chondrocytes. In conclusion, our findings establish a novel epigenomic mechanism of skeletal development, where Arid5b promotes chondrogenesis by facilitating a relationship between Sox9 and Phf2-mediated histone demethylation of chondrogenic gene promoters.

Sox 9是一种对软骨内骨化至关重要的转录因子;然而，其表观遗传调控的证据仍然难以捉摸。在这里，我们确定Arid 5 b（AT丰富的互动结构域5 b）作为一种新的转录辅助调节Sox 9。Arid 5 b与Sox 9相互作用，并通过直接结合Col 2a 1基因启动子协同促进软骨形成。Arid 5 b ^-/-小鼠表现出生长迟缓，软骨形成延迟。Arid 5 b ^-/-软骨细胞中软骨形成标记基因启动子H3 K9甲基化水平显著升高。总之，我们的研究结果建立了一种新的骨骼发育的表观基因组机制，其中Arid 5 b通过促进Sox 9和Phf 2介导的软骨形成基因启动子的组蛋白去甲基化之间的关系来促进软骨形成。

项目成果

転写因子FoxC1はPTHrPの発現誘導を介して内軟骨性骨形成を制御する

转录因子 FoxC1 通过诱导 PTHrP 表达来控制软骨内骨形成

• 发表时间: 2012
• 作者: 吉田倫子;波多賢二;高島利加子;井関祥子;山本照子;西村理行;米田俊之
• 通讯作者: 米田俊之

Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription.

• DOI: 10.1091/mbc.e10-07-0566
• 发表时间: 2011-04-15
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Amano K;Hata K;Muramatsu S;Wakabayashi M;Takigawa Y;Ono K;Nakanishi M;Takashima R;Kogo M;Matsuda A;Nishimura R;Yoneda T
• 通讯作者: Yoneda T

Regulation of endochondral ossification by transcription factors

• DOI: 10.2741/4076
• 发表时间: 2012-06
• 期刊: Frontiers in Bioscience
• 作者: R. Nishimura;K. Hata;K. Ono;K. Amano;Yoko Takigawa;M. Wakabayashi;R. Takashima;T. Yoneda

Spatiotemporal disorder in the axial skeleton development of the Mesp2-null mouse: a model of spondylocostal dysostosis and spondylothoracic dysostosis.

• DOI: 10.1016/j.bone.2012.11.033
• 发表时间: 2013-03
• 期刊: Bone
• 影响因子: 4.1
• 作者: Y. Makino;Yu Takahashi;R. Tanabe;Y. Tamamura;Takashi Watanabe;M. Haraikawa;M. Hamagaki;K. Hata;J. Kanno;T. Yoneda;Y. Saga;M. Goseki‐Sone;K. Kaneko;A. Yamaguchi;T. Iimura

The transcription factor Arid5b is a novel partner of Sox9 in endochondral bone formationR Takashima

转录因子 Arid5b 是软骨内骨形成中 Sox9 的新伙伴 R Takashima

• 发表时间: 2011
• 作者: K Hata;K Ono;M Wakabayashi;K Amano;M Nakanishi;Y Maeda;R Whitson,R Nishimura;T Yoneda
• 通讯作者: T Yoneda