Development of a novel system for monitoring cellular ATP using a unique enzyme reaction

在生物素化反应中，生物素蛋白连接酶(BPL)介导生物素与生物素羧基载体蛋白(BCCP)的特定赖氨酸残基的结合。喜温古生菌Sulfolobus tokodaii的生物素化反应有一个独特的特点，即酶BPL与其产物生物素化的BCCP形成稳定的络合物。在目前的工作中，我们开发了一种利用这种独特的生物素化反应来检测ATP的系统。

In the biotinylation reaction, biotin protein ligase (BPL) mediates the attachment of biotin to a specific lysine residue of biotin carboxyl carrier protein (BCCP). The biotinylation reaction from thermophilic archaeon Sulfolobus tokodaii has a unique characteristic that the enzyme BPL forms a stable complex with its product, biotinylated BCCP. In the present work, we have developed a detection system for ATP with this unique biotinylation reaction.