Regulation of protein function in living cells by using a unique enzyme reaction

古菌硫藻的生物素化反应有一个独特的特点，即生物素连接酶与其生物素化的底物蛋白形成稳定的复合物。在目前的工作中，我们试图利用这种独特的生物素化反应，构建一个能够调节活细胞中靶蛋白功能的系统。通过这项研究，我们成功地调控了活细胞中靶蛋白的定位。

Biotinylation reaction from archaeon Sulfolobus tokodaii has a unique characteristic that the biotin ligase forms a stable complex with its biotinylated substrate protein. In the present work, by using this unique biotinylation reaction, we attempted to construct the system that enables the regulation of the functions of the target proteins in living cells. As a result of the study , we have succeeded in the regulation of localization of a target protein in living cells.