Development of growth inhibition technology of toxic-producing blue-green algae

产毒蓝绿藻生长抑制技术开发

• 批准号: 19760370
• 负责人: HIDAKA Taira
• 金额: $ 2万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19760370/
• 关键词: 水質汚濁・土壌汚染防止・浄化; モデル化; 有害化学物質; 有害物質生成藻類; Microcystis属; 培養実験; リアルタイムPCR; 遺伝子; 検鏡; 水質; Microcystin

Applicability of real-time PCR method was evaluated by cultivation experiments of Microcystis. After logarithmic growth phase, quantification results by both mcy A gene and 16S rRNA gene were similar to cell numbers measured by microscopic analysis. Increase in cell number and mcy A gene corresponded with increase in microcystin production. Simultaneous increase in mcy A gene and microcystin was observed in a field survey. These results proved that real-time PCR quantification can be applied to microcystin-producing blue-green algae, and it is promising to develop monitoring and growth inhibition technologies of toxic-producing blue-green algae.

通过微囊藻的培养实验，评价实时荧光定量PCR方法的适用性。对数生长期结束后，mcy A基因和16S rRNA基因的定量结果与显微镜分析测得的细胞数量相似。细胞数量和mcya基因的增加与微囊藻毒素产量的增加相对应。在野外调查中观察到mcya基因和微囊藻毒素同时增加。这些结果证明，实时荧光定量PCR技术可以应用于产微囊藻毒素的蓝藻，开发产毒蓝藻的监测和生长抑制技术具有广阔的前景。

项目成果

Analysis of factors affecting the ratio of microcystin to chlorophyll‐a in cyanobacterial blooms using real‐time polymerase chain reaction

• DOI: 10.1002/tox.20525
• 发表时间: 2011-02
• 期刊: Environmental Toxicology
• 影响因子: 4.5
• 作者: J. Ha;T. Hidaka;H. Tsuno

リアルタイムPCRによるミクロシスチン生成藍藻類の分析

通过实时 PCR 分析产生微囊藻毒素的蓝藻

• 发表时间: 2009
• 作者: 日高平;河周賢;楠田育成;津野洋
• 通讯作者: 津野洋