Development of growth inhibition technology of toxic-producing blue-green algae

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Applicability of real-time PCR method was evaluated by cultivation experiments of Microcystis. After logarithmic growth phase, quantification results by both mcy A gene and 16S rRNA gene were similar to cell numbers measured by microscopic analysis. Increase in cell number and mcy A gene corresponded with increase in microcystin production. Simultaneous increase in mcy A gene and microcystin was observed in a field survey. These results proved that real-time PCR quantification can be applied to microcystin-producing blue-green algae, and it is promising to develop monitoring and growth inhibition technologies of toxic-producing blue-green algae.