Development of detection and inducing method for single cell apoptosis and study on blood mineralization with these method
Development of detection and inducing method for single cell apoptosis and study on blood mineralization with these method
批准号:
19770163
负责人:
KIHARA Takanori
金额:
$2.39万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009
中文摘要
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英文摘要
I have been developed the single cell apoptosis detection method using nanoprobe. The nanoprobe for enzyme or mRNA was newly constructed and I have succeeded in detection of apoptosis in a single cell by insertion the nanoprobe to the living cell. Moreover, I tried to develop the apoptosis inducing method in a single cell. As constructing the apoptosis ligand modified probe, I succeeded in detection of specific interaction between the apoptosis ligand and receptor on a single cell surface.
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Development of a caspase-3 probe functioned on the surface of nanoneedle to evaluate the enzyme activities in single living cell
开发在纳米针表面起作用的 caspase-3 探针以评估单个活细胞中的酶活性
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[KIHARA, Takanori, 木原隆典]
通讯作者:
木原隆典
Surface modification of nanoneedle with 2-methacryloyloxyethyl phosphorylchorine (MPC) polymers for improving the biocompatibility with cell interior
2-甲基丙烯酰氧基乙基磷酰氯(MPC)聚合物对纳米针进行表面修饰以改善与细胞内部的生物相容性
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[HIHARA, Takanori]
通讯作者:
Takanori
AFMを用いたバイオ分子・細胞の力学的計測
使用 AFM 对生物分子和细胞进行机械测量
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[KIHARA, Takanori, 三宅淳]
通讯作者:
三宅淳
ナノ針の細胞挿入効率に対する積層ナノ薄膜の効果
层压纳米薄膜对纳米针细胞插入效率的影响
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[MIEDA, Shingo, 中村史, 杉立年弘, 三枝真吾, 木原隆典, 金百合恵, 中村史, 河野景子]
通讯作者:
河野景子
Nanoneedle surface modification with 2-methacryloyloxyethyl phosphorylcholine polymer to reduce nonspecific protein adsorption in a living cell
用 2-甲基丙烯酰氧基乙基磷酸胆碱聚合物修饰纳米针表面以减少活细胞中的非特异性蛋白质吸附
DOI:
--
发表时间:
2007
期刊:
NanoBiotechnology 3
影响因子:
--
作者:
[Kihara, T., Yoshida, N., Mieda, S., Fukuzawa, K., Nakamura, C., Ishihara, K., Miyake, J.]
通讯作者:
J.
共 37 条
Analysis of physical properties of actin filaments during differentiation of smooth muscle cells
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批准号:24700454
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.83万
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财政年份:2012
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负责人:KIHARA Takanori
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依托单位:
海外基金