Analysis of physical properties of actin filaments during differentiation of smooth muscle cells

肌动蛋白细丝的动力学调控着平滑肌细胞的分化。在这项研究中，我检测了CRP2，一种平滑肌细胞相关基因的特异性转录辅助因子，对肌动蛋白细丝结构和物理性质的影响。用小角X射线散射分析证明了CRP2在溶液中的结构。CRP2通过与肌动蛋白细丝结合，增加肌动蛋白细丝的直径和持续长度。此外，CRP2的过表达增加了平滑肌细胞的力学性能。因此，我澄清了CRP2是一种调节溶液中肌动蛋白细丝结构和物理性质的因子。

Dynamics of actin filaments regulate differentiation of smooth muscle cells. In this study, I examined the effects of CRP2, which is a specific transcription cofactor for smooth muscle cell related genes, on structure and physical property of actin filaments. The structure of CRP2 in solution was demonstrated using small angle X-ray scattering analysis. CRP2 increased the diameter and the persistent length of the actin filaments by binding with actin filaments. Furthermore, the mechanical properties of smooth muscle cells were increased by overexpression of CRP2. Therefore, I clarified that CRP2 was a factor regulating the structure and physical property of actin filaments in solution.