An approach to establish a method that allows efficient screening of beneficial probiotic strains for livestock.

从“对肠道上皮细胞的影响”和“宿主特异性”两个方面评价益生菌的效果。本研究表明，大多数益生菌乳酸菌(LAB)在物理状态下对肠上皮细胞的基因表达无明显影响，但在炎症状态下对其基因表达无明显影响。此外，至少在脾细胞、肠系膜淋巴结组织与LAB的体外共培养体系中没有发现宿主特异性。因此，可以利用小鼠细胞筛选出对猪有效的益生菌菌株。

Effect of probiotics was evaluated with consideration of "effect on intestinal epithelial cells" and "host specificity". This study showed most probiotic lactic acid bacteria (LAB) did not affect considerably on gene expression of intestinal epithelial cells under physical condition but not inflammatory status. Additionally no host specificity was found at least in in vitro co-culture system of splenocyte, mesenteric lymph node tissue with LAB. Thus, probiotic strains effective on pigs could be screened by using mouse cells.