An approach to establish a method that allows efficient screening of beneficial probiotic strains for livestock.

一种建立有效筛选牲畜有益益生菌菌株的方法的方法。

• 批准号: 19780198
• 负责人: INOUE Ryo
• 金额: $ 2.47万
• 依托单位: Kyoto Prefectural University (2008-2009)Hokkaido University (2007)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19780198/
• 关键词: プロバイオティクス; 上皮細胞; ブタ; インターロイキン12; 腸管上皮細胞; Caco-2

Effect of probiotics was evaluated with consideration of "effect on intestinal epithelial cells" and "host specificity". This study showed most probiotic lactic acid bacteria (LAB) did not affect considerably on gene expression of intestinal epithelial cells under physical condition but not inflammatory status. Additionally no host specificity was found at least in in vitro co-culture system of splenocyte, mesenteric lymph node tissue with LAB. Thus, probiotic strains effective on pigs could be screened by using mouse cells.

从“对肠道上皮细胞的影响”和“宿主特异性”两个方面评价益生菌的效果。本研究表明，大多数益生菌乳酸菌(LAB)在物理状态下对肠上皮细胞的基因表达无明显影响，但在炎症状态下对其基因表达无明显影响。此外，至少在脾细胞、肠系膜淋巴结组织与LAB的体外共培养体系中没有发现宿主特异性。因此，可以利用小鼠细胞筛选出对猪有效的益生菌菌株。

项目成果

Diverse Immune Responses to Orally Administrated Heat-Killed Cell Preparation of Enterococcus faecalis Strain EC-12 in Immune Tissues in Mice.

小鼠免疫组织中粪肠球菌菌株 EC-12 口服热灭活细胞制剂的多种免疫反应。

• 发表时间: 2009
• 期刊: Bioscience Biotechnology and Biochemistry 73(6)
• 作者: Yoshikawa;T. Inoue;R. Yoshida;Y. Watanabe;T. Bukawa;W.;Ushida;K.
• 通讯作者: K.

乳酸菌のIL-12産生誘導能に関する研究

乳酸菌诱导IL-12产生能力的研究

• 发表时间: 2010
• 作者: 平井康丸;森 健;梛野崇之
• 通讯作者: 梛野崇之