Analysis of the relationship between nodule and plant by the positron-imaging

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In this study, we have developed a method to produce ^<13>N-labeled radioactive nitrogen gas (^<13>N_2) with high purity. We fed the ^<13>N_2 mixed with defined concentrations of O_2 and N_2 to the underground part of intact nodulated soybean plants and obtained movies of fixation of nitrogen gas using PETIS (positron-emitting tracer imaging system) successfully. The rates of fixation within 10 min were estimated quantitatively.