Intestinal immune system : its development and interaction with intestinal bacteria

肠道免疫系统：其发育及其与肠道细菌的相互作用

基本信息

批准号：
19780229
负责人：
SUZUKI Hodaka
金额：
$ 2.37万
依托单位：
National Institute of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

In the previous study, regional differences in intraepithelial lymphocytes (IELs) were studied in the small intestine of mice. In this study, further studies were performed on the basis of the previous findings and IELs number and subsets distributed in the entire intestine, including cecum and colon, were studied. Most of the significant differences in phenotypic compositions were found between the small and large intestines, although some differences were found among the different parts of the small and large intestines. These results indicate that the differences between IELs in the small and large intestines are discontinuous. Such regional differences in IELs of mouse intestines were similar from young adult to aged mice, although the number of IELs was highest at 6 months old and then gradually decreased with age. Such decrease of the lymphocytes may be a kind of aging symptoms in intestinal immune system. Regional differences in gene expression profiles of Peyer's patches (PP), which act as inductive sites of intestinal immune network, were also determined in mice. Of the 187 genes that were expressed differently among the PPs from different regions, 6 genes were related to immune system process. These findings suggest that the regional differences among PPs in mice in terms of the immunological activation status and/or immunological functions may be subtle in the normal state.

在先前的研究中，在小鼠小肠中研究了上皮内淋巴细胞（IEL）的区域差异。在这项研究中，研究了包括先前的发现和IELS数量和子集在整个肠道中分布在包括塞库姆和结肠在内的整个肠道中进行的进一步研究。在小肠和大肠之间发现了表型组合物的大多数显着差异，尽管在大小肠的不同部分中发现了一些差异。这些结果表明，小肠和大肠中的IEL之间的差异是不连续的。从年轻人到老年小鼠的IELS IELS的这种区域差异相似，尽管IEL的数量在6个月大，然后随着年龄的增长而逐渐减少。淋巴细胞的这种减少可能是肠道免疫系统中的一种衰老症状。在小鼠中也确定了PEYER斑块（PP）的基因表达谱（PP）的区域差异（PP）。在来自不同区域的PPS中表达不同的187个基因中，有6个基因与免疫系统过程有关。这些发现表明，就免疫激活状态和/或免疫功能而言，小鼠PPS之间的区域差异可能在正常状态下是微妙的。