Investigation for ovarian cancer therapy focusing on production of human chorionic gonadotropin beta subunit

以人绒毛膜促性腺激素β亚基产生为重点的卵巢癌治疗研究

• 批准号: 19591938
• 负责人: TASHIRO Hironori
• 金额: $ 2.91万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591938/
• 关键词: 婦人科腫瘍学; 細胞・組織; 遺伝子; 癌; 上皮性卵巣癌; 絨毛ゴナドトロピン; βサブユニット; LH; hCG受容体; 卵巣表層上皮; βサブニット; 卵巣表層上皮細胞

Most patients with ovarian cancer are diagnosed at an advanced stage. Although treatment for ovarian cancer consists of surgical treatment and combination chemotherapy, there is still poor prognosis associated with drug resistance. As an experimental model system for epithelial ovarian carcinogenesis, we established immortalized human ovarian surface epithelial (OSE) cell lines. Using this system, we clarified the production of hCG α subunit and β subunit in the OSE cells, and the effect of hCG β subunit in anchorage-independent growth of the OSE cells. Moreover, many ovarian cancer tissues also expressed hCG β subunit. This discovery will provide a development of new therapeutic strategies for ovarian cancer.

大多数卵巢癌患者在晚期被诊断出来。尽管卵巢癌的治疗包括手术治疗和联合化疗，但与耐药性相关的预后仍然较差。作为上皮性卵巢癌发生的实验模型系统，我们建立了永生化人卵巢表面上皮（OSE）细胞系。利用此系统，我们阐明了OSE细胞中hCG α亚基和β亚基的产生，以及hCG β亚基在OSE细胞非贴壁依赖性生长中的作用。此外，许多卵巢癌组织也表达hCG β亚基。这一发现将为卵巢癌的治疗提供新的策略。

项目成果

Long-term oncological outcomes of ovarian serous carcinomas with psammoma bodies : a novel insight to the molecular pathogenesis of ovarian epithelial carcinoma.

卵巢浆液性癌与砂粒体的长期肿瘤学结果：对卵巢上皮癌分子发病机制的新见解。

• 发表时间: 2010
• 期刊: Cancer Science
• 影响因子: 5.7
• 作者: T.Motohara;H.Tashiro;et al.
• 通讯作者: et al.

Neurofibromin 1 (NF1) Defects Are Common in Human Ovarian Serous Carcinomas and Co-occur with TP53 Mutations

• DOI: 10.1593/neo.08784
• 发表时间: 2008-12-01
• 期刊: NEOPLASIA
• 影响因子: 4.8
• 作者: Sangha, Navneet;Wu, Rong;Cho, Kathleen R.
• 通讯作者: Cho, Kathleen R.

Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes

• DOI: 10.1093/carcin/bgp007
• 发表时间: 2009-03-01
• 期刊: CARCINOGENESIS
• 影响因子: 4.7
• 作者: Sasaki, Rumi;Narisawa-Saito, Mako;Kiyono, Tohru
• 通讯作者: Kiyono, Tohru

The immunohistochemical analysis of 17B-hydroxysteroid dehydrogenase isozymes in human ovarian surface epithelium and epithelial ovarian carcinoma

人卵巢表面上皮和上皮性卵巢癌中17B-羟基类固醇脱氢酶同工酶的免疫组织化学分析

• 发表时间: 2010
• 期刊: Medical Molecular Morphology (In press)
• 作者: Motohara K.;et al.
• 通讯作者: et al.

Establishment of a model for epithelial ovarian cancer using normal human ovarian surface epithelial cells

正常人卵巢表面上皮细胞建立上皮性卵巢癌模型

• 发表时间: 2008
• 作者: Imai Y;Ohmori K;Yasuda S;Wada M;Suzuki T Fukuda K;Ueda Y;Sasaki R
• 通讯作者: Sasaki R